2010年山东师范大学生物化学考研试题.pdf
tlJ * yf!J m* * J1lJf91:1:A*iJiJRlJI : . 2. 3. * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * ilf=.J J9f f!) j.f .ff10f1:-1h x.? ;f:,;t f!) j.ft :11: 811 mfJ.ffI DJJGJtt? 1s,iA)!) iJP.1t,-.ft6 3-*1;K -i fJ JL1t 2.? (2 0 1J-) 2. M#?, #JL -k:r10f1iitr1I*. 8 ? (2 0 ) 3. i,f.:1i r9 -%r, :1i -1t !8J ffJ A4t, it!t4-8 ltL r I!J A E; T 1f3 A 4t6 I!J -iF, -itqH r 6 fJ i, 4-tI: G E; C C.,xt 8t 8 it 2 0 1J-)0 ( Adenine: thymine 5z-f q ., f)14 , A,B ff.Jj ill 1-11 Ij JL / . r1Ar7 /0 F- ;fer IJ 8 ! J:. ,/LineweaverBurk db t ( xx. 1f; t ), 1n1X, 5R L!l R fl;9( 8 f1Af*IJ ifJtt-ij; JJ ;+ *tJ.a-( 2 0:0-), -j;I 1;f,1, JJ1t ? A 1/Vo 1/ISJ B 1/VO 1/S v), T A, B, C :0IJIJ 6ry 1f-EJ J0 ii.iJfJW ,-itiJL 8J t!f11-IJIJ JLuJ#-*)1f-EJ J0 ii.Jif-iJf ;-? f;j i.it#7J ;-8 ;f. JW c ( 2 0:0) Porous beads Glass plastic column 6 -t-:Small molecules B 01 different prc!eins):1 Ugand ./ immobilizea on inSOluble support and packed in glass column c Mixture of proteins Positively charged beads Glass column POSitively charged proteins .,; I ,;fjP Tube to collect : protein.t. 6 Specific Soluble ligand protein added W -:proteins pass Specific / straight through protein elutes W oft bound to soluble ligand Buffer f- t+)O wiNegatively&Y cnarged r+.j:I proteins0 -_ t? ra00r Negati/ely r TUbesto101 charGed collect e-1 proteins o protein 0 o 0 6 , 5K Jt 6!:J f6 i 1 - f6*, *gffi: 11-jIJ Jl:.1t z.1(1c-iffit ( JfJ rf X-; ;f;f-*X-m J?; 1(1c-tJit; ;f;f-3:5j ! Of ) ? *-itoJ 1I-J.t i:tf 2 0 11-)0 ( 2nd base1st base 3rd base (5 end) U C (3 end) t A G t Phe Ser Tyr Cys U U Phe Ser Tyr Cys C Ser I A31Ser 14 I G 1 Pro His Arg U C Pro His Arg C Pro Gin Arg A Pro Gin Arg G lie Thr Asn Ser U A lie Thr Asn Ser C lie Thr Lys Arg A I 2 I Thr Lys Arg G Val Ala Asp Gly U G Val Ala Asp Gly C Val Ala Glu Gly A Val Ala Glu Gly G l-_., I i coo I 7, T fJ 7b +Jc J !iU1jt1mlr-tJR.B , -it1fJ:i*Jj Jc J!j #Zlffit1mJ 3 0 5J-)0 ( coo I CHzI HO-C-COO_ cooI CH2 I 2H2coo I H-C-COOI HO-C H c=o I I coo CH2 I cootJi 1fl B7fit, i1%1ft L ijilj 7B B !i fml.:F &.m NADH 0;1 : mffiR*iF fD 0 ? (fiijilJ 1llttit: EtL:7C ! ) NADH? ? ? o