生化进展精品文稿.ppt

生化进展课件第1页，本讲稿共19页Abstract1、Habronema muscae(Spirurida:Habronematidae)occurs in the stomach of equids,is transmitted by adult muscid dipterans and causes gastric habronemiasis2、Scanning electron microscopy(SEM)was used to study the morphological aspects of adult worms of this nematode in detail.第2页，本讲稿共19页INTRODUCTIONBackground：1.The adult worms live superficially,under the mucous layer,with only their heads buried in the mucosal spaces in the stomach.2.The main effect of Habronema in the stomach is to stimulate the secretion of large amounts of thick,stringy,tenacious mucus,with associated histological change consisting of hyper-plasia and hypertrophy of the mucus-secreting cells.Purpose：Although there have been studies evaluating the morphology of avian habronematid nematodes based on light and scanning electron microscopy,the present(SEM)study is the first to describe,in detail,H.muscae removed from a naturally infected horse.第3页，本讲稿共19页MATERIALS AND METHODSMaterials:A total of 36 worms(22 females and 14 males)were removed from the stomach of a 13-year-old horse.第4页，本讲稿共19页Methods:1.The worms were washed in 2%sodium cacodylate buffer(pH 7.2),and then cleared with lactophenol solution.2.Identification of the species was confirmed on the basis of light microscopic examination.Among examined worms,12 were identified as H.muscae(seven females and five males).3.The nematodes were put into fresh 4%glutarldehyde for 2 h,post fixed in 1%osmium tetraoxide in cacodylate buffer(pH 7.2)at 4C for 1 h and dehydrated through a series of increasing ethanol concentrations.第5页，本讲稿共19页4.They were then dried with liquid CO2 in a critical point drier apparatus.5.Each worm was placed on a numbered aluminium stab,which was coated with gold in an ioncoating sputter and viewed with a JEOL JSM-840 Scanning Electron Microscope operated at 15 kV.Photographs were taken with a digital acquisition system for analog SEMs.第6页，本讲稿共19页RESULTSFig.1 Scanning electron micrograph of the anterior end of the female Habronema muscae,showing sub-median cephalic papilla(arrowhead),cervical papilla(CP),the lateral ala(LA),and excretory pore(EP).Bar=10 mFig.2 Scanning electron micrograph of the anterior end of the female Habronema muscae,showing cephalic region with two lateral lips(Llip),each was trilobed(stars),four sub-median cephalic papillae(CP),and amphidal apertures(A).Bar=10 m第7页，本讲稿共19页Fig.3 Scanning electron micrograph of the anterior end of the female Habronema muscae,showing cephalic region with lateral lip(Llip),which was trilobed(stars),sub-median cephalic papillae(CP),and amphidal aperture(A).Bar=10 mFig.4 Scanning electron micrograph of the anterior end of the male Habronema muscae,showing cervical papilla(CP).Bar=10 m第8页，本讲稿共19页Fig.5 Scanning electron micrograph of the anterior end of the male Habronema muscae,showing excretory pore(EP),and cuticular transverse striations(TS).Bar=10 mFig.6 Scanning electron micrograph of the anterior end of the male Habronema muscae,showing abnormal feature,with lateral lip which was trilobed(stars),sub-median cephalic papillae(CP)and amphidal apertures(A).Bar=10 m第9页，本讲稿共19页Fig.7 Scanning electron micrograph of the female Habronema muscae,showing vulva(V),and cuticular transverse striations(TS).Bar=10 mFig.8 Scanning electron micrograph of the female Habronema muscae,showing anal pore(AP)and phasmid(Ph).Bar=100 m第10页，本讲稿共19页Fig.9 Scanning electron micrograph of the posterior end of the female Habronema muscae,showing anal pore(AP).Bar=10 mFig.10 Scanning electron micrograph of the posterior end of the female Habronema muscae,showing a higher magnification of phasmids(Ph).Bar=10 m第11页，本讲稿共19页Fig.11 Scanning electron micrograph of the posterior end of the male Habronema muscae,showing the caudal alae(CA),spicule(S)and cuticular ridges(CR).Bar=100 mFig.12 Scanning electron micrograph of the posterior end of the male Habronema muscae,showing pre-cloacal papillae(PrCP),spicule(S),postcloacal papillae(PoCP),and cuticular ridges(CR).Bar=100 m第12页，本讲稿共19页Fig.13 Scanning electron micrograph of the posterior end of the male Habronema muscae,showing precloacal papillae(PrCP),and cuticular ridges(CR).Bar=10 mFig.14 Scanning electron micrograph of the posterior end of the male Habronema muscae,showing the cloaca(star),the unpaired postcloacal papillae,one on the right hand side(RPoCP)and two on the left hand side(LPoCP).Bar=10 m第13页，本讲稿共19页Fig.15 Scanning electron micrograph of the posterior end of the male Habronema muscae,showing a higher magnification of the unpaired postcloacal papillae,one on the right hand side(RPoCP)and two on the left hand side(LPoCP),cuticular ridges(CR),and cuticular bosses(CB).Bar=10 mFig.16 Scanning electron micrograph of the posterior end of the male Habronema muscae,showing a higher magnification of the cluster of small postcloacal papillae(arrowheads),phasmids(Ph),and cuticular ridges(CR).Bar=10 m第14页，本讲稿共19页DISCUSSIONThis paper describes,for the first time,most surface features of adult H.muscae(Spirurida,Habronematidae),removed from naturally infected horse by SEM.Another SEM study,which was carried out on Viguiera dicrurusi(Spirurida:Habronematidae)harboured by black drongoes.第15页，本讲稿共19页谢谢Make Presentation much more fun第16页，本讲稿共19页Title第17页，本讲稿共19页Title第18页，本讲稿共19页第19页，本讲稿共19页