实验九生长素类物质对水稻根芽生长的不同影响1教学内容.ppt

实验九生长素类物质对水稻根芽生长的不同影响1实验原理实验原理生长素是第一个被发现的植物激素，对植物生长有很大影响，其作用体现浓度效应：对某器官而言，低浓度表现促进效应；高浓度起抑制作用，不同的植物或同一植物的不同器官，对生长素的浓度反应都有差异。因此，生长素类物质对器官生长有一个最佳促进浓度。不同器官对生长素的敏感性不同，因此最佳促进浓度不同，促进生长的程度亦不同。一般根对生长素的敏感程度要比芽大得多，所以，根对生长素所要求的最适浓度要比芽低得多。萘乙酸（NAA）是一类人工合成的生长素类物质，对根、芽生长的不同影响与生长素一致。本实验将观测萘乙酸的不同浓度对水稻在根、芽生长上的不同影响。11/11/20222实验材料和器材实验材料和器材器材：恒温箱，9cm培养皿10套，9cm圆形滤纸10张，10 mL移液管2支、l mL移液管1支，镊子1把，直尺1把 试剂：10mg/L NAA 实验材料水稻种子11/11/20223实验步骤实验步骤种子培养：取大小一致的试材种子100粒，每皿10粒，沿培养皿四周均匀地播开加盖后放入25恒温箱中黑暗培养3天。测定：取出各号皿中种子，量取各粒种子的根数、根长，芽长。记入下表并计算平均数。配制NAA浓度梯度：给10套培养皿分别编号，在号皿中加9mL10mg/LNAA溶液；在号皿中加1mL10mg/LNAA，再加9mL蒸馏水，配成1mg/LNAA；从号皿中吸取1mL于号皿中，加9mL蒸馏水，配成0.1mg/LNAA液；依次配制梯度浓度，使至号皿中NAA浓度分别为；10、1、10-1、10-2、10-3、10-4、10-5、10-6、10-7mg/L，从最后的号皿中吸去1mL溶液。号皿中加入9mL蒸馏水作对照。在每个皿中加入一张圆形滤纸。11/11/20224实验报告实验报告1、为何要从9号皿中吸去1 mL溶液？2、在该实验中，如果培养皿的盖子盖得不严，有水分挥发，对实验结果有何影响？3、在该实验中使用移液管时，应注意些什么 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4G8JbNeQhTl11/11/20226此课件下载可自行编辑修改，仅供参考！此课件下载可自行编辑修改，仅供参考！感谢您的支持，我们努力做得更好！谢谢感谢您的支持，我们努力做得更好！谢谢