北大神经生物学课件2绪论ppt课件.ppt
北大神经生物学课件2绪论ppt课件 Still waters run deep.流静水深流静水深,人静心深人静心深 Where there is life,there is hope。有生命必有希望。有生命必有希望Effect of HANS on heart rate of heroin addictsDay of treatment1234567891060708090100110120130Control2 Hz2/100 Hz100 Hzn=13-16*Heart Rate(beat/min)Body Weight(kg)01234567891011Days of Treatment45505560Control2Hz2/100Hz100Hz(n=26-32)Effect of HANS on Body Weight during Opiate Withdrawal*The dose of The dose of BPNBPN needed by Heroin Addicts needed by Heroin Addicts during detoxification during detoxificationBPN Dose(BPN Dose(mg/dmg/d)1 12 23 34 45 56 67 78 89 91010 11111212 1313 1414Days of Treatment Days of Treatment (n=14)(n=14)0.00.00.50.51.01.01.51.52.02.02.52.5*BuprenorphineBuprenorphine HANS+BPN HANS+BPN *P0.001,compared with BPN group *P0.001,compared with BPN group CRAVING:the most difficult issue一朝吸毒,终身想毒。1 Month 1 Month0 05050100100Relapse Rate(%)Relapse Rate(%)*P0.05,compared with BPN group *P0.05,compared with BPN group 92%92%78%78%57%57%71%71%100%100%100%100%HANS Decreases Heroin Relapse Rate of the Heroin addicts 2 Month 2 Month 3 Month 3 MonthBPN(n=12)BPN(n=12)HANS+BPN(n=14)HANS+BPN(n=14)*The auto-track conditioned place preference(CPP)instrumentMorphine-induced conditioned place preference(CPP)Dose-dependent effect*p0.05,*p0.01,*p 100 Hz100 Hz 2 HzElectroacupuncture treatment for drug addictionVTAOpiateGABANAcDADATCocaineEuphoriaEnkDynElectroacupuncture2 Hz100 HzPresynaptic inhibitionSubstitutionA100 bp2000 bp1000 bp750 bp500 bp250 bpDL 2000 NSCPP2 Hz100 HzrestrainGAPDH(532 bp)PPD(250 bp)Effect of 100Hz EA on the level of PPD mRNA in NAc of CPP rats B0.00.51.01.5Grey scale ratios(PPD/GAPDH)Treatment*#n=4NSCPP2 Hz100 HzrestrainEffect of 2Hz EA on the level of PPE mRNA in NAc of CPP rats Grey scale ratios(PPE/GAPDH)0.000.250.500.751.00NSCPP*#2 Hz100 Hzrestrainn=42000 bp1000 bp750 bp500 bp250 bp100 bpDL2000CPP2 Hz100 HzrestrainGAPDH(532 bp)PPE(402 bp)NS美国权威性美国权威性“物质滥用物质滥用”教科书教科书Joyce Lowinson 等主编等主编第第4版,版,2004“针刺戒毒针刺戒毒”一章一章由韩济生编写由韩济生编写肥肥 胖胖 症症能量代谢失衡能量代谢失衡对美食的渴求对美食的渴求药物成瘾药物成瘾身体依赖:戒断症状身体依赖:戒断症状精神依赖:心瘾精神依赖:心瘾痛与镇痛痛与镇痛感知功能感知功能情绪反应情绪反应肥胖的发展趋势肥胖的发展趋势肥胖发病机理肥胖发病机理GenesEnvironmental factors HANSHANS治疗肥胖症:人体研究结果治疗肥胖症:人体研究结果048121620242832-4-3-2-10 A HANS B C HANS Dn=16Time(week)ABCD-4-3-2-10*HANS(3 time/week)Change of body weight(kg)梁门,一对。天枢,一对。水道,一对。梁丘,公孙。频率:2/100 Hz每周3次每次30 分钟 大鼠实验结果(高能食物引起肥胖)大鼠实验结果(高能食物引起肥胖)Diet-induced Obesity(DIO)After 14 weeks02468101214160100200300400500600700High energy diet(n=80)Normal diet(n=20)*Time(week)Body weight(g)ControlDRDIO0100200300400500600700800*#High energy dietBody weight(g)*P0.001 vs.control and DR;#P0.05 vs.Control促进摄食和抑制摄食的神经肽促进摄食 feedingNPY,Orexin,AGRP,MCH抑制摄食 feedingLeptin,CART,a-MSH,CRHNPYOrexinAGRPMCHCARTLeptin-MSH Insulin瘦胖ThinObese100Hz036912151821242730Time(day)9092949698100102104106108110Body weight (%)DIO大鼠实验结果(体重大鼠实验结果(体重%)Restrain2Hz大鼠实验结果(体重差值)大鼠实验结果(体重差值)*P0.001 vs.DIO;#p0.01,#P0.001 vs.RestrictionDIORestrain100 Hz2 Hz大鼠实验结果(大鼠实验结果(ACTHACTH)Normal DR DIO Restrain 100Hz EA 2Hz EA051015202530*High energy diet*Plasma ACTH (ng/ml)*P0.05 vs.Normal,DR and DIO.大鼠实验结果(能量摄取)大鼠实验结果(能量摄取)*P0.001 vs.other groups;#P0.001 vs.2Hz and 100 Hz EA group CART -MSHControlDiet induced ObesityDiet ResistantRestrain100Hz2HzCART immunoreactivity in hypothalamusControlDiet induced ObesityDiet ResistantRestrain100 Hz 2Hz-MSH immunoreactivity in hypothalamus大鼠实验结果(免疫组化)大鼠实验结果(免疫组化)n=5NormalDRDIORestrain100Hz EA0255075100125 *High energy diet CART positive cells in ARCHigh energy dietNormalDRDIORestrain100Hz EA2Hz EA010203040506070*a-MSH positive cells in ARC *2Hz EACART-MSH 国际神经科学神经科学领域:英国TiNS杂志(2003)国际针灸针灸领域:德国SEIRIN针灸奖(2002)国际戒毒戒毒理论:美国“物质成瘾”经典教科书 临床戒毒戒毒实践:韩氏戒毒一年以上不复吸20例36年针灸研究,12年戒毒研究所获成绩Trends in Neuroscience,2003;26:17-22 International SIERIN Acupuncture Award 2002Dusseldorf,Germany 美国权威性美国权威性“物质滥用物质滥用”教科书教科书Joyce Lowinson等主编等主编第第4版,版,2004“针刺戒毒针刺戒毒”一章一章由韩济生编写由韩济生编写