方穗山羊草Rubisco活性及小亚基基因克隆和功能分析_郭.pdf

植物学报?的?,势?一?注曰的丑角明血口?阮比口方穗山羊草?活性及小亚基基因克隆和功能分析郭绍东“吴光耀吴相枉“?北京大学生命科学学院,北京?猖?摘要以方穗山羊草?月吧放娜?为母本,普通小麦?阮?喇?为父本杂交,并以普 通小麦为父本回交?代获得 的核质杂种小麦 为实验材 料,测定了父母本及其核质杂种小麦?业石?的梭化活性和加氧活性。同时以方穗山羊草幼苗叶片为材料构建了库容量为?护画的碑?文库,并以水稻?三?部分片段为探针筛选该?文库,获得了方穗山羊草成?的?克隆?。核昔 酸序列分析表明该?全长?饰。比较以上三种材 料 中凡山?活性和小 亚基氨基 酸的差异,推测小亚基上第?、?、?和?位氨基酸残基可能对酶的功能起着重要的作用。关键词方穗山羊草,核质杂种小麦,?二。小亚基?习?侧匕?竹?工?肛?义汀污?刃?伙认?面?“,叭?耀一?!兀?一?关“?公泌伙卯?匀仑?,八石月?穷,?巧吨?仪阳?加。,?讨?,?州及塑?,了减忿?一?加?面?场?舍?杭?占,“朋飞招?早?板?刃?,?即?,?娜?川巧?护内?州?叮“。?”。贺?叮?祀?衍?卿?,?到?奴?习?甲】?】?巧坤?一?,?一?幻xyl*/o x yg ell巴犯(nlbis c o)伙朋A.阿aw it ht ha tf rol llT.a e sta n dt h ei rn ude o祀yt叩la s而chy-b r idwhea ta le dt ha tt h eal llin oa cid比siduesa tp ositions56,8 4,98an11 17int hesm al ls,一I卜俪t而g h tp城I ln l闪r ta lltr o l esi n,邵da t in gt h ea ct i v it i eso fn山10.价y wo八如A吧i阮哪sgu aa,Nucl e O一cyIDpleh声r idw heat,Sll l al ls ubunit)fribulo s e一1,5-b is曲osPlla t eearl刃xyla s e/。x og eR e ceiv(,1:1望灭i,(犯一0 5I kvi”卫:1性粥巧e s0 8一1 9Al止.a tio n s:骆U.Sn创1subu nit:烧U.1谈比ge5 1山朋it;R l,B I,.1亏11)。l、一I,5一bis P 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