软骨细胞增殖和迁移中力生长因子的影响,细胞生物学论文.docx
软骨细胞增殖和迁移中力生长因子的影响,细胞生物学论文摘要：背景:力生长因子在多种组织中表示出,能够促进多种组织细胞的增殖和迁移。目的:观察力生长因子对原代培养兔软骨细胞增殖和迁移的影响,以及内在机制讨论。方式方法:取北京市维通利华实验动物技术有限公司提供的新西兰大白兔的膝关节软骨,剪碎后,采用酶消化法原代分离培养软骨细胞,取处于对数生长期开放获取文章,根据(知识分享许可协议署名-非商业性使用-一样方式分享4.0条款,在合理引用的情况下,允许别人以非商业性目的基于原文内容编辑、调整和扩展,同时允许任何用户阅读、下载、拷贝、传递、打印、检索、超级链接该文献,并为之建立索引,用作软件的输入数据或其他任何合法用处。文题释义:力生长因子:是由胰岛素样生长因子基因编码的,在组织或者细胞处于应激和损伤修复时,通过对mRNA进行剪接编码而翻译产生的选择性剪接变异体蛋白,力生长因子能够有效促进肌肉细胞增殖,诱导成肌细胞向肌管细胞分化,同样可以以促进成骨细胞和韧带细胞的增殖和诱导分化。转化生长因子1/Smad信号通路:转化生长因子1信号通路介入生物体细胞的生长和分化经过,包括细胞生长、细胞分化、细胞凋亡、细胞动态平衡等其他细胞功能。转化生长因子1信号通路经过是:相关信号因子与型受体结合,型受体招募并磷酸化型受体,型受体再磷酸化受体调控的SMAD蛋白,这些蛋白再与SMAD4结合。再磷酸化受体调控的SMAD蛋白/SMAD4复合体作为转录因子在细胞核内聚集,介入目的基因表示出的调控。以下为参考文献：1MathenyRWJr,NindlBC,AdamoML.Minireview:Mechano-growthfactor:aputativeproductofIGF-Igeneexpressioninvolvedintissuerepairandregeneration.Endocrinology.2018;151(3):865-875.2PhilippouA,MaridakiM,HalapasA,etal.Theroleoftheinsulin-likegrowthfactor1(IGF-1)inskeletalmusclephysiology.InVivo.2007;21(1):45-54.3GoldspinkG.Mechanicalsignals,IGF-Igenesplicing,andmuscleadaptation.Physiology(Bethesda).2005;20:232-238.4Varela-EirinM,LoureiroJ,FonsecaE,etal.Cartilageregenerationandageing:Targetingcellularplasticityinosteoarthritis.AgeingResRev.2021;42:56-71.5LoeserRF,CollinsJA,DiekmanBO.Ageingandthepathogenesisofosteoarthritis.NatRevRheumatol.2021;12(7):412-420.6KobayashiT,FujitaK,KamataniT,etal.A-674563increaseschondrocytemarkerexpressioninculturedchondrocytesbyinhibitingSox9degradation.BiochemBiophysResCommun.2021;495(1):1468-1475.7MaN,WangH,XuX,etal.Autologous-cell-derived,tissue-engineeredcartilageforrepairingarticularcartilagelesionsintheknee:studyprotocolforarandomizedcontrolledtrial.Trials.2021;18(1):519.8KaraarslanN,BatmazAG,YilmazI,etal.Effectofnaproxenonproliferationanddifferentiationofprimarycellculturesisolatedfromhumancartilagetissue.ExpTherMed.2021;16(3):1647-1654.9ChenJJ,ZhangW.HighexpressionofWWP1predictspoorprognosisandassociateswithtumorprogressioninhumancolorectalcancer.AmJCancerRes.2021;8(2):256-265.10DavidE,TirodeF,BaudhuinM,etal.OncostatinMisagrowthfactorforEwingsarcoma.AmJPathol.2020;181(5):1782-1795.11KungLH,RajparMH,BriggsMD,etal.Hypertrophicchondrocyteshavealimitedcapacitytocopewithincreasesinendoplasmicreticulumstresswithouttriggeringtheunfoldedproteinresponse.JHistochemCytochem.2020;60(10):734-748.12PengC,ZhaoH,SongY,etal.SHCBP1promotessynovialsarcomacellmetastasisviatargetingTGF-1/Smadsignalingpathwayandisassociatedwithpoorprognosis.JExpClinCancerRes.2021;36(1):141.13ZhaoH,PengC,RuanG,etal.Adenovirus-deliveredPDCD5counteractsadriamycinresistanceofosteosarcomacellsthroughenhancingapoptosisandinhibitingPgp.IntJClinExpMed.2020;7(12):5429-5436.14ArmakolasA,PhilippouA,PanteleakouZ,etal.PreferentialexpressionofIGF-1Ec(MGF)transcriptincanceroustissuesofhumanprostate:evidenceforanovelandautonomousgrowthfactoractivityofMGFEpeptideinhumanprostatecancercells.Prostate.2018;70(11):1233-1242.15DengM,ZhangB,WangK,etal.MechanogrowthfactorEpeptidepromotesosteoblastsproliferationandbone-defecthealinginrabbits.IntOrthop.2018;35(7):1099-1106.16QinLL,LiXK,XuJ,etal.Mechanogrowthfactor(MGF)promotesproliferationandinhibitsdifferentiationofporcinesatellitecells(PSCs)bydown-regulationofkeymyogenictranscriptionalfactors.MolCellBiochem.2020;370(1-2):221-230.17李元靖,刘欢,樊欣,等.MGF对人软骨终板干细胞增殖和迁移的影响J.中国细胞生物学学报,2021,37(3):335-342.18ShaY,YangL,LvY.ERK1/2andAktphosphorylationwereessentialforMGFEpeptideregulatingcellmorphologyandmobilitybutnotproangiogeniccapacityofBMSCsunderseverehypoxia.CellBiochemFunct.2021;36(3):155-165.19ZhangB,LuoQ,SunJ,etal.MGFenhancestenocyteinvasionthroughMMP-2activityviatheFAK-ERK1/2pathway.WoundRepairRegen.2021;23(3):394-402.20LuoZ,JiangL,XuY,etal.Mechanogrowthfactor(MGF)andtransforminggrowthfactor(TGF)-3functionalizedsilkscaffoldsenhancearticularhyalinecartilageregenerationinrabbitmodel.Biomaterials.2021;52:463-475.21LiC,VuK,HazelgroveK,etal.IncreasedIGF-IEcexpressionandmechano-growthfactorproductioninintestinalmuscleoffibrostenoticCrohnsdiseaseandsmoothmusclehypertrophy.AmJPhysiolGastrointestLiverPhysiol.2021;309(11):G888-899.22JingX,YeY,BaoY,etal.Mechano-growthfactorprotectsagainstmechanicaloverloadinduceddamageandpromotesmigrationofgrowthplatechondrocytesthroughRhoA/YAPpathway.ExpCellRes.2021;366(2):81-91.23HeJ,DongL,XuK,etal.MechanogrowthfactorEpeptideinhibitsinvasionofmelanomacellsandup-regulatesCHOPexpressionviaendoplasmicreticulumstress.BiotechnolLett.2021;40(1):205-213.24LiH,LeiM,YuC,etal.Mechanogrowthfactor-Eregulatesapoptosisandinflammatoryresponsesinfibroblast-likesynoviocytesofkneeosteoarthritis.IntOrthop.2021;39(12):2503-2509.25TongY,FengW,WuY,etal.Mechano-growthfactoracceleratestheproliferationandosteogenicdifferentiationofrabbitmesenchymalstemcellsthroughthePI3K/AKTpathway.BMCBiochem.2021;16:1.26DengM,LiuP,XiaoH,etal.ImprovingtheosteogenicefficacyofBMP2withmechanogrowthfactorbyregulatingthesignalingeventsinBMPpathway.CellTissueRes.2021;361(3):723-731.27LuoQ,WuK,ZhangB,etal.MechanogrowthfactorEpeptidepromotesratbonemarrow-derivedmesenchymalstemcellmigrationt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