microRNAs-的重要性课件.ppt

Importance of microRNA for seedling development in Arabidopsis thaliana Jennifer CoppersmithDepartment of Biochemistry and BiophysicsMentor:Dr.Hiro Nonogaki(Horticulture)Significance of Seed Developmentpgrc3.agr.ca/images/seeds.gif Agriculture and Food Why Arabidopsis?Arabidopsis Seed StructureEmbryoEndospermTestaOverview of ExperimentT DNA insertSQUAMOSA PROMOTER-BINDING PROTEIN-LIKE13(mSPL13)SPL13RISCRISCmiRNAmicroRNAmRNAmiRNARISCmiRNAmicroRNA mutationmRNAMutationHypothesis:SPL13 needs to be down regulated by microRNA156 to maintain normal developmental program for germination or seedling establishment.AAAASPL13miR156mSPL13 WT Cont mSPL13rRNASPL13Overaccumulation of SPL132-leaf seedlings4-leaf seedlingsDevelopmental DelayTemporal Seedling Arrest WTmSPL13CONTROLStorage Protein and MeristemMeristemEmbryoCotHypocotylRadicalmSPL13WT8 dVisible DifferenceAnalysis WTmSPL13SPL13Based on phenotype,down regulation of SPL13 via microRNA is essential for proper meristem development.AcknowledgementsHiro NonogakiKevin AhernHoward Hughes Medical InstituteLab Members:Masa AsahinaTammy ChanPo-Pu Liu Jessica KristofPiotr PupelJing SunSQUASQUAMOSA(or APETALA1)10 bp conserved stretch19-mer core seq-419-393-455-429AP1SBP1SBP2SPL13?Normal Embryogenesis in mSPL13Coppersmith JL,Hewitt JR,Nonogaki H,unpublished results