(完整word版)生物化学技术习题册答案(word文档良心出品)39439.pdf

1.2.3.4.5.6.Gel Chromatography)1.B A.B.C.D.E 2.C A.206nm B 240nm C 260nm D280nm E 304nm 3.260nmE A.B.C.D.E.4.D A.206nm B 240nm C260nm D280nm E 304nm 5.DA.220nm B 245nm C 260nm D 280nm E 340nm 6.DNAAA.C ug/ml=A260X 50 X B.C ug/ml=A260X 40 X C.C ug/ml=A260X 30 X D.C ug/ml=A260X 20 X E.C ug/ml=A260X 10 X 7.DNA(B A.C ug/ml=A260X 50 X B.C ug/ml=A260X 40 X C.C ug/ml=A260X 30 X D.C ug/ml=A260X 20 X E.C ug/ml=A260X 10X 8.RNA(B A.C ug/ml=A260X 50 x B.C ug/ml=A260X 40 x C.C ug/ml=A260X 30 x D.C ug/ml=A260X 20 x E.C ug/ml=A260X 10 x 9.DNA(C A.A260/A280=1.4 B.A260/A280=1.6 C.A260/A280=1.8 D.A260/A280=2.0 E.A260/A280=2.2 10.RNA(D A.A260/A280=1.4 B.A260/A280=1.6 CA260/A280=1.8 D.A260/A280=2.0 E.A260/A280=2.2 11.DNADNA20A260nm=0.44A280nm=0.251cmDNA(D A.250g/ml B.264 g/ml C.352 g/ml D.440 g/ml E.220 g/ml 12.(C A.B C D E 13.(D A.B C D E 14.(C A.B C D E 15.(C A.PAGEB.IEFC.SDS-PAGED.E.SDS-PAGEPAGE IEF16.(D A.B.C.D.E.17.(A A.B.C.D.E.18.C A.B.C.D.E.1.2.3.1-1 4-4.5.6.7.1.）2.3.,4.5.1.10%A D A.0.01 B.0.1 C.0.1D.1E.22.1%A E A.0.001 B.0.01 C.0.2D.1 E.3.200400nmC A.B.C.D.E.4.400760nmB A.B.C.D.E.5.ATD A.BC E 1-T 6.2mm 4mm 6mm 8mmC AB C.x2x3x4xD.xx/2x/3x/4 E7.C A.BCD.E8.D A.B C.D.E.9.,C A.B CD E 10.630nm C A B CD E1.（,）pH2.,.,，LamberBeer,3.，:e11cm.A=e cLE1%1%（w/v 1cm.A=E1%cLCAME AGE 1.2.3.（Mobility）M=V/E=Q/6n r n4.（-COOH）（-NH2）pH，pH5.6.7.1.A A.B.a 1-C.a 1-D.3-E.-2.E A.B.a C.a D.3-E.-3.E A.B.a 1-C.a 1-D.3-E.-4.A A.B.a 1-C.a 1-D.3-E.-5.A A.a-B.3-C.3-D.6.D A.a-B.3-C.3-D.7.D A.a-B.3-C.3-D.8.A A.a-B.3-C.3-D.9.pI 4.65.05.36.77.3pH A A.7.0B.6.0C.8.6D.4.0E.3.010.pH pI 4.65.0A.6.0B.5.0C.4.0D.3.0E.2.011.pH8.6 B A 5v/1 cm B20 v/1cm C8 v/1cm D15 v/1cm E12 v/1cm 12.pH8.6 A A0.02M B0.03M C0.04M D0.05M E0.06M 13.pH 4.96.8E ApH8.6 BpH6.5 CpH4.9 DpH6.8 EpH5.85 1 2U，,3M=V/E=Q/6n r n。4pH;5CAMEpH8.6pI,，CAM，5 6B，pH8.6，PAGE IEF 1.N,N-2.100ml Acr Bis 3.BisAcrBis4.pH1.C A.PAGE B.SDS-PAG C.IEF D.CAME E.AGE 2.IEF D A.C.3.IEF D A.C.4.PAGE B.D.BD.A.T B.T C.T D.T 5.B A.B.C.D.6.PAGE TEMED A A.B.C.D.7.D A.B.C.D.8.A A.B.C.D.9.PAGE AP B A.B.C.D.10.-TEMED C A B C D E11.B A TEMED B CD E12.D A BpHC D E13.Ampholine D A BCD pHE1PAGacrylamide Acr N,N-N,N,-methylene bisacrylamide Bis AP,TEMED2.100ml Acr Bis BisAcrBis3.pH 4.pH A.B.C.D.51.：2.：3.：，4.：，56.7.8.9.，10.Ve:1.BA.Ve=Vo Kd=0 B.Ve=Vi Kd=1 C.Ve=Vo+Vi Kd=1 D.0:Kd：1 Ve=Vo+Kd Vi 2.DA.Kd=1 Ve=Vo B.Kd=0 Ve=Vi D.0:Kd：1Ve=Vo+Kd Vi 3.Bio-Gel P-100DA.100 B.1000 C.10000 4.KdBA.KLB.KLC.KL5.DA.B.C.6.Kd=0.25Kd=0.75AA.B.7.Sephadex G50CA.0.05ml B.0.5ml C.5ml D.50ml 8.Sephadex G75B A.0.75ml B.7.5ml C.7.5L 9.BpHC.Kd=0 Ve=Vo+Vi D.100000 D.K L D.C.D.D.75ml 10.A A.B.C.D.A.B.C.D.12CA.PAGE B.IEF C.D.13CA B CD E.14X Y ZSephadex G-25YZ D A ZB ZCZDZ X EZ Y 1,2,3,4Kd=（Ve-V0）/Vi 561,2.:（）11Sephadex G50A Ve Ve 3.:,4.:,5.6.inwvIV,1.Dowex50 pH6.0B A.pl 9.74B.pl 2.77C.pl 10.76 D.pl5.962.C A.B.C.D.3.A A B CD E.4pl=9.74pl=5.48 pI=10.76 pl=3.22 pH5.38 C A-B-C-D-E-5.D A 420nm B 480nm C540nm D570nm M E650nm6.DNS-CIDNS-A A.a-B.3-C.Y-D.S-7.A A.IB.nC D.W8.D A.IB.nC D.W1,2（）,3,4,（）,5DNS-DNS-CIDNS,.,DNA PCR（PCR）DNA.DNA107108DNA1.DNA DNA 1 0A260nm=0.44A280nm=0.251cmDNAD A.250g/ml B.132 g/ml C.176 g/ml D.220 g/ml 2.DNA DNA Tm D A.DNA A-T 15%B.DNA G-C 25%C.DNA G-C 40%D.DNA A-T 80%3.PCR C 2+AMg B.C.RNAD.DNA 4.PCR D A.B.C.D.Taq DNA5.DNAA A.B.C.D.6.DNA B A.B.C.D.7.DNA D A.B.DNA C.DNA RNA D.DNA 8.DNA-DNA A A.B.C.D.9.DNA-B AB.C.D10.DNA C ADNA B.CD.DNAE.DNA 11.Tm A A DNA260nmTmB.Tm 50%C.Tm G+C D.DNASE.DNA 12.B A DNAB.DNAC.DNA D.DNA E.DNA 13.DNA A A.G+C Tm B.A+T Tm C.Tm=A+T+G+CD.DNA Tm E.DNA 14.DNA C A.DNase B.DNA C.D.RNase 1.DNA DNADNADNA DNA DNA DNA DNASDSDNA-DNA2.DNA DNA-，；5mol/L NaCI DNA DNA DNA 70%DNA,TE DNA 3.PCRPolymerase chain reaction PCRDNA PCRDNAMg2+4dNTPTaq DNA94 CDNADNA72 CTaq DNA5 34 dNTP DNA DNA 2530 DNA 106 4 DNA DNA（g/ml）=A260nm x 50Xx 1/DNA（g）=DNAxDNA5DNA,，pH410，,