中医方证代谢组学.docx

中医方证代谢组学 打开文本图片集 摘要证候和方剂是中医学的2个关键科学问题，干脆关系到中医疾病的诊断和临床治疗的有效性。方剂是中医临床用药的主要形式和手段，其配伍的科学性和临床的有效性是中医药科学价值的详细体现。然而，中医证候的模糊性及方剂的困难性极大地限制了方剂的效应评价及中药药效物质基础的确认。如何解决证候的诊断标准，以及相关的方剂药效物质基础的确认是国际关注的热点问题，迫切须要建立符合其自身特点的探讨方法和技术体系。中医方证代谢组学是将中药血清药物化学和代谢组学有机结合，在解决证候生物标记物的基础上，建立方剂药效生物评价体系，进而发觉与临床疗效干脆相关的药效物质基础，阐明作用机制的方法学体系。中医方证代谢组学将“中医证候生物标记物-方剂体内干脆作用物质-药效生物标记物”探讨有机结合，建立血清中外源性中药成分与内源性标记物群2组变量相关分析方法，评价方剂整体效应及方证相应关系，揭示方剂的药效物质基础及其配伍规律的应用探讨策略;该策略的实施将促进方剂向化学成分明确化、作用机制清楚化的方向发展，为方剂关键科学问题探讨带来方法学创新，有助推动中医方-证相关探讨，揭示生命本质并为人类健康做出有益贡献。 关键词中医方证代谢组学;中药血清药物化学;代谢组学;方剂;药效物质基础;证候;生物标记物 Chinmedomics： a new strategy for research of traditional Chinese medicine ZHANG Ai-hua， SUN Hui， YAN Guang-li， WANG Ping， HAN Ying， WANG Xi-jun* AbstractSyndrome and formulae are two key issues in traditional Chinese medicine and the premise research for material basis of TCM. However， vagueness of syndromes and complexity of formulae greatly limited the evaluation to syndromes and effective substance basis of prescription. Therefore， how to solve the evaluation of syndromes， confirming the efficacy material basis in prescription are the current hot issues of international concern. To solve these problems， establishing chinmedomics by integrated serum pharmacochemistry of TCM with metabolomics technology， that is a unique method of TCM research， made outstanding contributions in solving international concerns such as the effectiveness and security aspects of TCM. On the basis of the biological characterization of syndrome， the metabolic profiling of animal models of TCM syndrome， and related metabolic fingerprints as well as metabolic biomarkers were established to evaluate the overall effects of TCM formulae and corresponding relationship of syndrome-formulae. The active constituents were screened using the plotting of correlation between marker metabolites and serum constituents ， and is ongoing verification by further biological experiments. Correlation analysis between the ingredients in the body after oral formulae and endogenous markers in vivo can be used to clarify the active ingredients and synergistic properties. This method was successfully applied for rapid discovery of potentially bioactive components and metabolites from TCM， and through a series of studies on the chinmedomics， it proved that the established method could help to explore the effective substance for further research of TCM. As a new research approach， Chinmedomics is the best method to fit the holistic concept of TCM， and it can not only interpret the essence of syndrome but also elucidate the scientific connotation of Chinese medical formulae. 第4页 共4页第 4 页 共 4 页第 4 页 共 4 页第 4 页 共 4 页第 4 页 共 4 页第 4 页 共 4 页第 4 页 共 4 页第 4 页 共 4 页第 4 页 共 4 页第 4 页 共 4 页第 4 页 共 4 页