邹3.pptx

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1、Lecture3Proteinsortingamongcellularcompartments,Overviewofmajorprotein-sortingpathwaysineukaryotes,transmembranetransportgatedtransportvesiculartransport,StructureoftheroughER,ExperimentshowingthatsecretoryproteinsentertheER,Translationandtranslocationoccursimultaneously,Cotranslationaltranslocation

2、,Signalrecognitionparticle:sixproteinsplusa300ntRNA,Sec61aisatransloconcomponent,energycomesfrompeptidetranslocationduringtranslation,Post-translationaltranslocationneedsATPhydrolysisbyBiP,ERmembraneproteins,PositioningtypeIsingle-passproteins,topogenicsequences:signalsequence,stop-transferanchorseq

3、uenceandsignal-anchorsequence,PositioningtypeIIandtypeIIIsingle-passproteins,Insertionoftail-anchoredprotein.,TopogenicsequencesdetermineorientationofERmembraneproteins,GPI-anchoredproteins,Biosynthesisoftheoligosaccharideprecursor,AdditionandinitialprocessingofNlinkedoligosaccharides,Actionofprotei

4、ndisulfideisomerase(PDI),Theunfolded-proteinresponse,Overviewofmajorprotein-sortingpathwaysineukaryotes,Proteinimportintothemitochondrialmatrix,amphipathicN-terminalsignalsequenceTOMandTIMunfoldedproteinsaretransportedsignalsequenceiscleavedthreeenergyinputs,Threepathwaystotheinnermitochondrialmembranefromthecytosol,Twopathwaystothemitochondrialintermembranespace,Transportingproteinstochloroplastthylakoids,useprotongradient,Overviewofmajorprotein-sortingpathwaysineukaryotes,