从质谱数据鉴定多肽蛋白质ppt课件.ppt

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1、中科院自动化所 李伏欣Peak m/z: 1019.08DFPIANGER 1019.09EPISVSSQQMLK 1347.56VLDALDSIK 974.13Carbonic anhydrase II SHHWGYGKHBGPZHWHKDFPIANGERQSPVNIDTKAVVQDPALKPLALVYGEATSRRMVNNGHSFNVEYDDSQDKAVLKDGPLTGTYRLVQFHFHWGSSBBQGSEHTVDRKKYAAELHLVHWNTKYGDFGTAAQQPDGLAVVGVFLKVGDANPALQKVLDALDSIKTKGKSTDFPNFDPGSLLPNVLDYWTYPGSLT

2、TPPLLESVTWIVLKEPISVSSQQMLKFRTLNFNAEGEPELLMLANWRPAQPLKNRQVRGFPK多匹配可以大大降低随机匹配的概率,从而增加结果的可信度Molecular weights used for calculations.ButtoncodeShortcodeNameAverage massof residueMonoisotopic mass of residueTwenty Naturally-Occurring Amino AcidsAlaAAlanine71.078871.03711CysCCysteine103.1448103.00919AspDA

3、spartic Acid115.0886115.02694GluEGlutamic Acid129.1155129.04259PheFPhenylalanine147.1766147.06841GlyGGlycine57.052057.02146HisHHistidine137.1412137.05891IleIIsoleucine113.1595113.08406LysKLysine128.1742128.09496LeuLLeucine113.1595113.08406MetMMethionine131.1986131.04049AsnNAsparagine114.1039114.0429

4、3ProPProline97.116797.05276GlnQGlutamine128.1308128.05858ArgRArginine156.1876156.10111SerSSerine87.078287.03203ThrTThreonine101.1051101.04768ValVValine99.132699.06841TrpWTryptophan186.2133186.07931TyrYTyrosine163.1760163.06333dtytxyxC)()(),(10),(niiyixyxC质谱图De-novo Sequencing序列数据库一级质谱二级质谱质量纹数据库检索后筛选蛋白质未知多肽PSMs谢谢大家！