限制性内切酶保护碱基(5页).doc

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1、-限制性内切酶保护碱基寡核苷酸序列切割率%2 hr20 hrAcc I GGTCGACC CGGTCGACCG CCGGTCGACCGG 0 0 0 0 0 0 Afl III CACATGTG CCACATGTGG CCCACATGTGGG 0 90 90 0 90 90 Asc I GGCGCGCC AGGCGCGCCT TTGGCGCGCCAA 90 90 90 90 90 90 Ava I CCCCGGGG CCCCCGGGGG TCCCCCGGGGGA 50 90 90 90 90 90BamH I CGGATCCG CGGGATCCCG CGCGGATCCGCG 10 90 90

2、25 90 90 Bgl II CAGATCTG GAAGATCTTC GGAAGATCTTCC 0 75 25 0 90 90 BssH II GGCGCGCC AGGCGCGCCT TTGGCGCGCCAA 0 0 50 0 0 90 BstE II GGGT(A/T)ACCC 0 10 BstX I AACTGCAGAACCAATGCATTGG AAAACTGCAGCCAATGCATTGGAA CTGCAGAACCAATGCATTGGATGCAT 0 25 25 0 50 90Cla I CATCGATG GATCGATC CCATCGATGG CCCATCGATGGG 0 0 90 5

3、0 0 0 90 50 EcoR I GGAATTCC CGGAATTCCG CCGGAATTCCGG 90 90 90 90 90 90 Hae III GGGGCCCC AGCGGCCGCT TTGCGGCCGCAA 90 90 90 90 90 90 Hind III CAAGCTTG CCAAGCTTGG CCCAAGCTTGGG 0 0 10 0 0 75 Kpn I GGGTACCC GGGGTACCCC CGGGGTACCCCG 0 90 90 0 90 90Mlu I GACGCGTC CGACGCGTCG 0 25 0 50 Nco I CCCATGGG CATGCCATGG

4、CATG 0 50 0 75 Nde I CCATATGG CCCATATGGG CGCCATATGGCG GGGTTTCATATGAAACCC GGAATTCCATATGGAATTCC GGGAATTCCATATGGAATTCCC 0 0 0 0 75 75 0 0 0 0 90 90 Nhe I GGCTAGCC CGGCTAGCCG CTAGCTAGCTAG 0 10 10 0 25 50 Not ITTGCGGCCGCAA ATTTGCGGCCGCTTTA AAATATGCGGCCGCTATAAA ATAAGAATGCGGCCGCTAAACTAT AAGGAAAAAAGCGGCCGCA

5、AAAGGAAAA0 10 10 25 250 10 10 90 90Nsi ITGCATGCATGCA CCAATGCATTGGTTCTGCAGTT10 9090 90Pac ITTAATTAA GTTAATTAAC CCTTAATTAAGG0 0 00 25 90Pme IGTTTAAAC GGTTTAAACC GGGTTTAAACCC AGCTTTGTTTAAACGGCGCGCCGG0 0 0 750 25 50 90Pst IGCTGCAGC TGCACTGCAGTGCA AACTGCAGAACCAATGCATTGG AAAACTGCAGCCAATGCATTGGAA CTGCAGAAC

6、CAATGCATTGGATGCAT0 10 90 90 00 10 90 90 0Pvu ICCGATCGG ATCGATCGAT TCGCGATCGCGA0 10 00 25 10Sac ICGAGCTCG1010Sac IIGCCGCGGC TCCCCGCGGGGA0 500 90Sal IGTCGACGTCAAAAGGCCATAGCGGCCGC GCGTCGACGTCTTGGCCATAGCGGCCGCGG ACGCGTCGACGTCGGCCATAGCGGCCGCGGAA0 10 100 50 75Sca IGAGTACTC AAAAGTACTTTT10 7525 75Sma ICCCGG

7、G CCCCGGGG CCCCCGGGGG TCCCCCGGGGGA0 0 10 9010 10 50 90Spe IGACTAGTC GGACTAGTCC CGGACTAGTCCG CTAGACTAGTCTAG10 10 0 090 90 50 50Sph IGGCATGCC CATGCATGCATG ACATGCATGCATGT0 0 100 25 50Stu IAAGGCCTT GAAGGCCTTC AAAAGGCCTTTT90 90 9090 90 90Xba ICTCTAGAG GCTCTAGAGC TGCTCTAGAGCA CTAGTCTAGACTAG0 90 75 750 90 90 90Xho ICCTCGAGG CCCTCGAGGG CCGCTCGAGCGG0 10 100 25 75Xma ICCCCGGGG CCCCCGGGGG CCCCCCGGGGGG TCCCCCCGGGGGGA0 25 50 900 75 90 90-第 5 页酶