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1、AatII识别位点GACGTC CTGCAG Acc65I识别位点GTMKAC CAKMTG AccI识别位点GTMKAC CAKMTG AciI识别位点CCGC GGCG AclI识别位点AACGTT TTGCAA AcuI识别位点CTGAAG GACTTC AfeI识别位点AGCGCT TCGCGA AflII识别位点CTTAAG GAATTC AflIII识别位点ACRYGT TGYRCA AgeI识别位点ACCGGT TGGCCA AhdI识别位点GACNNNNNGTC CTGNNNNNCAG AleI识别位点CACNNNNGTG GTGNNNNCAC AluI识别位点AGCT TCG

2、A AlwI识别位点GGATC CCTAG AlwNI识别位点CAGNNNCTG GTCNNNGAC ApaI识别位点GGGCCC CCCGGG ApaLI识别位点GTGCAC CACGTG ApeKI识别位点GCWGC 名师资料总结-精品资料欢迎下载-名师精心整理-第 1 页，共 14 页 -CGWCG ApoI识别位点RAATTY YTTAAR AscI识别位点GGCGCGCC CCGCGCGG AseI识别位点ATTAAT TAATTA AsiSI识别位点GCGATCGC CGCTAGCG AvaI识别位点CYCGRG GRGCYC AvaII识别位点GGWCC CCWGG AvrII识

3、别位点CCTAGG GGATCC BaeI识别位点NACNGTAYCN BamHI识别位点GGATCC CCTAGG BanI识别位点GGYRCC CCRYGG BanII识别位点GRGCYC CYCGRG BbsI识别位点GAAGAC CTTCTG BbvCI识别位点CCTCAGC GGAGTCG BbvI识别位点GCAGC CGTCG BccI识别位点CCATC GGTAG BceAI识别位点ACGGC TGCCGBcgI识别位点CGANTGN GCTNACG 名师资料总结-精品资料欢迎下载-名师精心整理-第 2 页，共 14 页 -BciVI识别位点GTATCC CATAGG BclI识

4、别位点TGATCA ACTACT BfaI识别位点CTAG GATC BfuAI识别位点ACCTGC TGGACG BglI识别位点GCCNNNNNGGC CGGNNNNNCCG BglII识别位点AGATCT TCTAGA BlpI识别位点GCTNAGC CGANTCG Bme1580I识别位点GKGCMC CMCGKG BmgBI识别位点CACGTC GTGCAG BmrI识别位点ACTGGGN TGACCCN BmtI识别位点GCTAGC CGATCG BpmI识别位点CTGGAGN GACCTCN Bpu10I识别位点CCTNAGC GGANTCG BpuEI识别位点CTTGAGN G

5、AACTCN BsaAI识别位点YACGTR RTGCAY BsaBI识别位点GATNNNNATC CTANNNNTAG BsaHI识别位点GRCGYC CYGCRG 名师资料总结-精品资料欢迎下载-名师精心整理-第 3 页，共 14 页 -BsaI识别位点GGTCTCN CCAGAGN BsaJI识别位点CCNNGG GGNNCC BsaWI识别位点WCCGGW WGGCCW BsaXI识别位点NACNCTCCN NTGNGAGGN BseRI识别位点GAGGAGN CTCCTCN BseYI识别位点CCCAGC GGGTCG BsgI识别位点GTGCAG CACGTC BsiEI识别位点C

6、GRYCG GCYRGC BsiHKAI识别位点GWGCWC CWCGWG BsiWI识别位点CGTACG GCATGC BslI识别位点CCNNNNNNNGG GGNNNNNNNCC BsmAI识别位点GTCTCN CAGAGN BsmBI识别位点CGTCTCN GCAGAGN BsmFI识别位点GGGAC CCCTG BsmI识别位点GAATGCN CTTACGN BsoBI识别位点CYCGRG GRGCYC Bsp1286I识别位点GDGCHC CHCGDG 名师资料总结-精品资料欢迎下载-名师精心整理-第 4 页，共 14 页 -BspCNI识别位点CTCAGN GAGTCN BspD

7、I识别位点ATCGAT TAGCTA BspEI识别位点TCCGGA AGGCCT BspHI识别位点TCATGA AGTACT BspMI识别位点ACCTGCN TGGACGN BspQI识别位点GCTCTTCN CGAGAAGN BsrBI识别位点CCGCTC GGCGAG BsrDI识别位点GCAATGNN CGTTACNN BsrFI识别位点RCCGGY YGGCCR BsrGI识别位点TGTACA ACATGT BsrI识别位点ACTGGN TGACCN BssHII识别位点GCGCGC CGCGCG BssKI识别位点CCNGG GGNCC BssSI识别位点CACGAG GTGC

8、TC BstAPI识别位点GCANNNNNTGC CGTNNNNNACG BstBI识别位点TTCGAA AAGCTT BstEII识别位点GGTNACC CCANTGG 名师资料总结-精品资料欢迎下载-名师精心整理-第 5 页，共 14 页 -BstNI识别位点CCWGG GGWCC BstUI识别位点CGCG GCGC BstXI识别位点CCANNNNNNTGG GGTNNNNNNACC BstYI识别位点RGATCY YCTAGR BstZ17I识别位点GTATAC CATATG Bsu36I识别位点CCTNAGG GGANTCC BtgI识别位点CCRYGG GGYRCC BtgZI识

9、别位点GCGATG CGCTAC BtsCI识别位点GCAGTGNN CGTCACNN BtsI识别位点GCAGTGNN CGTCACNN Cac8I识别位点GCNNGC CGNNCG ClaI识别位点ATCGAT TAGCTA CspCI识别位点NCAANGTGGN NGTTNCACCN CviAII识别位点CATG GTAC CviKI-1识别位点RGCY YCGR CviQI识别位点GTAC CATG DdeI识别位点CTNAG GANTC 名师资料总结-精品资料欢迎下载-名师精心整理-第 6 页，共 14 页 -DpnI识别位点GATC CTAG DpnII识别位点GATC CTAG

10、DraI识别位点TTTAAA AAATTT DraIII识别位点CACNNNGTG GTGNNNCAC DrdI识别位点GACNNNNNNGTC CTGNNNNNNCAG EaeI识别位点YGGCCR RCCGGY EagI识别位点CGGCCG GCCGGC EarI识别位点CTCTTC GAGAAG EciI识别位点GGCGGA CCGCCT EcoNI识别位点CCTNNNNNAGG GGANNNNNTCC EcoO109I识别位点RGGNCCY YCCNGGR EcoP15I识别位点CAGCAG GTCGTC EcoRI识别位点GAATTC CTTAAG EcoRV识别位点GATATC C

11、TATAG FatI识别位点CATG GTAC FauI识别位点CCCGC GGGCG 名师资料总结-精品资料欢迎下载-名师精心整理-第 7 页，共 14 页 -Fnu4HI识别位点GCNGC CGBCG FokI识别位点GGATGN CCTACN FseI识别位点GGCCGGCC CCGGCCGG FspI识别位点TGCGCA ACGCGT HaeII识别位点RGCGCY YCGCGR HaeIII HgaI识别位点GACGC CTGCG HhaI识别位点GCGC CGCG HincII识别位点GTYRAC CARYTG HindIII识别位点AAGCTT TTCGAA HinfI识别位点G

12、ANTC CTNAG HinP1I识别位点GCGC CGCG HpaI识别位点GTTAAC CAATTG HpaII识别位点CCGG GGCC HphI识别位点GGTGA CCACT Hpy188I识别位点TCNGA AGNCT Hpy188III识别位点TCNNGA AGNNCT Hpy99I识别位点CGWCG GCWGC 名师资料总结-精品资料欢迎下载-名师精心整理-第 8 页，共 14 页 -HpyAV识别位点CCTTC GGAAG HpyCH4III识别位点ACNGT TGCCA HpyCH4IV HpyCH4V识别位点TGCA ACGT KasI识别位点GGCGCC CCGCGG K

13、pnI识别位点GGTACC CCATGG MboI识别位点GATC CTAG MboII识别位点GAAGA CTTCT MfeI识别位点CAATTG GTTAAC MluI识别位点ACGCGT TGCGCA MlyI识别位点GAGTC CTCAG MmeI识别位点TCCRAC AGGYTG MnlI识别位点CCTC GGAG MscI识别位点TGGCCA ACCGGT MseI识别位点TTAA AATT MslI识别位点CAYNNNNRTG GTRNNNNYAC MspA1I识别位点CMGCKG GKCGMC MspI识别位点CCGG GGCC 名师资料总结-精品资料欢迎下载-名师精心整理-第

14、 9 页，共 14 页 -MwoI识别位点GCNNNNNNNGC CGNNNNNNNCG NaeI识别位点GCCGC CGGCCG NarI识别位点GGCGCC CCGCGG NciI识别位点CCSGG GGSCC NcoI识别位点CCATGG GGTACC NdeI识别位点CATATG GTATAC NgoMIV识别位点GCCGGC CGGCCG NheI识别位点GCTAGC CGATCG NlaIII识别位点CATG GTAC NlaIV识别位点GGNNCC CCNNGG NmeAIII识别位点GCCGAG CGGCTC NotI识别位点GCGGCCGC CGCCGGCG NruI识别位点

15、TCGCGA AGCGCT NsiI识别位点ATGCAT TACGTA NspI识别位点RCATGY YGTACR PacI识别位点TTAATTAA AATTAATT PaeR7I识别位点CTCGAG GAGCTC 名师资料总结-精品资料欢迎下载-名师精心整理-第 10 页，共 14 页 -PciI识别位点ACATGT TGTACA PflFI识别位点GACNNNGTC CTGNNNCAG PflMI识别位点CCANNNNNTGG GGTNNNNNACC PhoI识别位点GGCC PleI识别位点GAGTCN CTCAGN PmeI识别位点GTTTAAAC CAAATTTG PmlI识别位点C

16、ACGTG GTGCAC PpuMI识别位点RGGWCCY YCCWGGR PshAI识别位点GACNNNNGTC CTGNNNNCAG PsiI识别位点TTATAA AATATT PspGI识别位点CCWGG GGWCC PspOMI识别位点GGGCCC CCCGGG PspXI识别位点VCTCGAGB BGAGCTCV PstI识别位点CTGCAG GACGTC PvuI识别位点CGATCG GCTAGC PvuII识别位点CAGCTG GTCGAC RsaI识别位点GTAC CATG 名师资料总结-精品资料欢迎下载-名师精心整理-第 11 页，共 14 页 -RsrII识别位点CGGWC

17、CG GCCWGGC SacI识别位点GAGCTC CTCGAG SacII识别位点CCGCGG GGCGCC SalI识别位点GTCGAC CAGCTG SapI识别位点GCTCTTCN CGAGAAGN Sau3AI识别位点GATC CTAG Sau96I识别位点GGNCC CCNGG SbfI识别位点CCTGCAGG GGACGTCC ScaI识别位点AGTACT TCATGA ScrFI识别位点CCNGG GGNCC SexAI识别位点ACCWGGT TGGWCCA SfaNI识别位点GCATCN CGTAGN SfcI识别位点CTRYAG GAYRTC SfiI识别位点GGCCNNN

18、NNGGCC CCGGNNNNNCCGG SfoI识别位点GGCGCC CCGCGG SgrAI识别位点CRCCGGYG GYGGCCRC SmaI识别位点CCCGGG GGGCCC 名师资料总结-精品资料欢迎下载-名师精心整理-第 12 页，共 14 页 -SmlI识别位点CTYRAG GARYTC SnaBI识别位点TACGTA ATGCAT SpeI识别位点ACTAGT TGATCA SphI识别位点GCATGC CGTACG SspI识别位点AATATT TTATAA StuI识别位点AGGCCT TCCGGA StyD4I识别位点CCNGG GGNCC StyI识别位点GCWWGG

19、GGWWCC SwaI识别位点ATTTAAAT TAAATTTA Taq I识别位点TCGA AGCT TfiI识别位点GAWTC CTWAG TliI识别位点CTCGAG GAGCTC TseI识别位点GCWGC CGWCG Tsp45I识别位点GTSAC CASTG Tsp509I识别位点AATT TTAA TspMI识别位点CCCGGG GGGCCC TspRI识别位点NNCASTGNN NNGTSACNN 名师资料总结-精品资料欢迎下载-名师精心整理-第 13 页，共 14 页 -Tth111I识别位点GACNNNGTC CTGNNNCAG XbaI识别位点TCTAGA AGATCT XcmI识别位点CCANNNNNNNNNTGG GGTNNNNNNNNNACC XhoI识别位点CTCGAG GAGCTC XmaI识别位点CCCGGG GGGCCC XmnI识别位点GAANNNNTTC CTTNNNNAAG ZraI识别位点GACGTC CTGCAG 与大家共享（非原创）名师资料总结-精品资料欢迎下载-名师精心整理-第 14 页，共 14 页 -