Abnova权威科普：Abnova重组蛋白NOG（人）中英文说明书.docx

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1、Abnova权威科普：Abnova重组蛋白NOG （人）中英文说明书一、艾美捷Abnova重组蛋白NOG （人）中文说明书中文名称：NOG （人）重组蛋白货号：P7011产品描述：在大肠杆菌中表达的C末端带有多组氨酸标签的人NOG重组蛋白。宿主：大肠杆菌形态：冻干制备方法：大肠杆菌表达系统纯化：Ni-NTA色谱法纯度：经SDS-PAGE测定98%内毒素水平：通过LAL方法，每lug蛋白质O.1EU。活性:通过其抑制ATDC5细胞产生BMP-4诱导的碱性磷酸酶的能力来衡量。在存在50 ng/mL重组人BMP-4的情况下，此效应的ED 500.05 ug/mLo质控检测：考马斯蓝SDS-PAGE染

2、色P7011的QC测试如下列图：75-63-48 2S17-11-推荐用法：SDS-PAGE,最正确工作稀释度应由最终用户确定。储存缓冲液：PBS冻干，pH 7.4o贮存说明：在-2CTC下贮存，冻干蛋白稳定1年。用去离子水重新组合后，在-20至-8（TC下 储存。等分以防止重复冷冻和解冻。应用说明：NOG (Human) Recombinant Protein (ug/mL) 通过其抑制ATDC5细胞产生BMP-4诱导的碱性磷酸酶的能力来衡量。在存在50 ng/mL重组 人BMP-4的情况下，这种作用的ED5098% bySDS-PAGEEndotoxin Level: 0.1 EU per

3、 1 ug of the protein by the LAL method.Activity:Measure by its ability to inhibit BMP-4-induced alkaline phosphatase production by ATDC5 cells. The ED 50 for this effect is 0.05 ug/mL in the presence of 50 ng/mL of recombinant human BMP-4.Quality Control Testing:SDS-PAGE Stained with Coomassie Blue7

4、5- 63-48 35-2517-11-Recommend Usage:SDS-PAGE； The optimal working dilution should be determined by the end user.Storage Buffer:Lyophilized from PBS, pH 7.4.Storage lnstruction:Store at -20, lyophilized protein is stable for 1 year.After reconstitution with deionized water, store at -20 to -80.Aliquo

5、t to avoid repeated freezing and thawing.Applications (Functional Study)NOG (Human) Recombinant Protein (ug/mL)Measure by its ability to inhibit BMP-4-induced alkaline phosphatase production by ATDC5 cells. The ED50 for this effect is 0.05 ug/mL in the presence of 50 ng/mL of recombinant human BMP-4.以上就是对于Abnova重组蛋白NOG （人）的中英文说明书，资料来源于艾美捷科技。