四川大学教案【首页】BasicBiomolecularExperiments.doc

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1、四 川 大 学 教 案【首页】课程名称Basic Biomolecular Experiments授课专业班级Class课程编号50128640课程类型必修课校级公共课（ ）；基础或专业基础课（）；专业课（ ）选修课限选课（ ）；任选课（ ）授课方式课堂讲授（ ）；实践课（）考核方式考试（）；考查（ ）课程教学总学时数68 hours学 分 数4学时分配课堂讲授 学时； 实践课 学时教材名称Basic Biomolecular Experiment textbook作 者Zhai Chaoyang出版社及出版时间2004指定参考书Medical Experiments of Molecular

2、 Biology Medical Immunological Experiments作 者Qing YangLi Mingyuan出版社及出版时间授课教师职 称Associated Professor and Lecturer单 位School of Preclinical and Forensic Medicine授课时间2005-2006注：表中（ ）选项请打“”四 川 大 学 教 案【医 科】周 次第 4 周， 第 1 次课 2006年 3 月 3 日备 注章 节名 称The principles of instruments,such as autoclave, electronic

3、balance,spectrophotometer, centrifuge and electrophoresis.授 课方 式理论课（）；实践课（）；实习（）教学时数6 hours教学目的及要求1. Knowing the principles of instruments in the experiments, particularly the major equipments in important experiments.2. Expounding mechanism and features of experiment instruments,such as electrophor

4、esis instruments,spectrophotometer,centrifuge,et al.3. Learning essentiality and fundamentalitys in the experiments.4. Request each university man to operate the instruments all by himself.教学 主 要内容时间分配1. Explain the assignment of this course2. The introduction of the primary instruments and techniqu

5、es in the process of experiments3. Introduce the report format of this experiment course,how to write a perfect reports4. Teachers have many claims on cleanness and sanitation5. Instructor illustrates and operate the instruments6. University men operate the instruments all alone10min50min10min5min60

6、min第页教学重点与难点1.The introduction of the primary instruments and techniques in the process of experiments2.The report format s of this experiment course,how to write a perfect reports in our laboratory3. Instructor illustrates and operate the instruments4. University men operate the instruments all alo

7、ne备 注启发提问1. What is the principle of centrifuge and how to run?2. why is the balance essential before centrifugation?3. please explain the principles of electrophoresis according to your knowledge.4. Present a proposal about determination of protein concentration in biochemistry experiment.外语要求1. Ma

8、ster basic dialogue of special nomenclature in biochemistry and molecular biology and immunology in experiments2. Narrate the necessary principles in English教学手段1. Discription on blackboard2. Questioning and answering3. Teaching demonstration参考资料注：教案按授课次数填写，每次授课均应填写一份。重复班授课可不另填写教案。生物分子 实 验 教 案周 次第 5

9、 周， 第 2 次课 2006年 3 月23 日备 注章 节名 称Determination of Blood Glucose and Influence of Hormone on Blood Glucose授 课方 式理论课（）；实践课（）；实习（）教学时数6 hours教学目的及要求1.Mastering influence of hormones on blood glucose2.Learning the determination of hormones in blood glucose(Wu Xian-Folin Methods)3.Clinical signifance of

10、blood glucose measurement4.Understanding the different functions of insulin and adrenalin in glycose metabolism,learning of the regulatory mechanism in organism.5.Knowing well the use of spectrophotometer6.Mastering the Operation of experimental animals.教学 主 要内容时间分配1. Importance of hormones in blood

11、 glucose constant in organism and its physiological significance.2. The Interplay and function of hormones such as insulin, glucagons,glucocorticoids etc.3. The methods of blood glucose determination4. Teaching manifestation of experimental animals5. Metabolism of blood glucose and influence and reg

12、ulation of hormones on blood glucose in normal level.6. The calculation the concentration of blood glucose 7. University men do experiments all by themselves and instructor direct them to operate10min10min10min50min30min10min23hrs第一页教学重点与难点1.Blood glucose regulation of hormones such as Insulin,Gluca

13、gons,Glucocorticoids etc2.The principles of blood glucose determination(Wu Xian-Folin Methods)3.The calculation of blood glucose concentration4.The regulatory mechanism of hormones in blood glucose level备 注启发提问1. Oral presentation of the physiological significance of blood glucose constant2. The glu

14、cose metabolism and hormone regulation外语要求1. Master basic dialogue of operation in experiments2. Narrate the necessary principles of hormone regulation教学手段1. Because of limited class time, no much time to present significance of the glucose metabolism2. Discription on blackboard3. Teaching manifesta

15、tion参考资料无注：教案按授课次数填写，每次授课均应填写一份。重复班授课可不另填写教案。四 川 大 学 教 案【医 科】周 次第 6 周， 第 3 次课 2006年 3 月 30 日备 注章 节名 称Electrophoresis of Serum授 课方 式理论课（）；实践课（）；实习（）教学时数6 hours教学目的及要求1. Knowing the principles of instruments in the experiments, particularly the major equipments in this experiments.2. The electrophores

16、is principles and features of experimental equipments.3. Learning the operation steps of Cellulose acetate electrophoresis and disc electrophoresis.4. Understanding the basic mechanism of protein band staining in electrophoresis.5. Mastering the key operation steps in electrophoresis教学 主 要内容时间分配1. N

17、arrate the principles and methods of electrophoresis techniques,and applications of isolation and purification in medical biochemistry and molecular biology.2. Narrate the isolation of serum protein and its significance in immunology.3. Expound the detailed operation of cellulose acetate electrophor

18、esis,the technique of adding sample.4. Expound the electrophoresis medium of polyacrylamide gel electrophoresis ( PAGE ) and its principle(disc electrophoresis) in isolation.5. Tell university men to pay attention to the observation and recording of the results.6. Complete the experiment operation t

19、hemselves30min10min10min10min20min60min第页教学重点与难点1.The operation and the principles of electrophoresis2.The preparation of Gel in disc electrophoresis and taking out the gel from the tubes;Adding the sample in the cellulose acetate electrophoresis3.The detailed operation of cellulose acetate electrop

20、horesis,the technique of adding sample.4. Expound the electrophoresis medium of polyacrylamide gel electrophoresis ( PAGE ) and its principle(disc electrophoresis) in isolation.备 注启发提问1. How much do you learn about the principles of electrophoresis?2. Do you know the mechanism of electrophoresis sep

21、aration?3. How to observe the results of electrophoresis isolation外语要求1. Master the major terms in electrophoresis2. Narrate the necessary mechanism of electrophoresis3. Narrate common concepts and principles in English.4. Pronounce well and truly,and expound clearly and reposefully5. Teaching thoug

22、ht is legible,and university men understand easily.教学手段1.Blackboard writing and multiple media2.Connecting with clinical testing and examples3.Questions参考资料无注：教案按授课次数填写，每次授课均应填写一份。重复班授课可不另填写教案。生物分子实验教案周 次第 7 周， 第4次课 2006年 4 月 6 日备 注章 节名 称Basic Immunological Experiments:Agglutination and Precipitatio

23、n Reactions;Countercurrent Immunoelectrophoresis,etc.授 课方 式理论课（）；实践课（）；实习（）教学时数6 hours教学目的及要求1. Mastering the methods of blood type characterization,and some concepts and principles2. Knowing well antibody-antigen reactions: precipitation,agglutination,coagulation.3. Understanding the immunodiffusio

24、n and immunoelectrophoresis4. Learning the operation of tube agglutination reaction5. Learning to observe the experiment results教学 主 要内容时间分配1.The basic concepts:antigen,antibody,serology, precipitation reaction,agglutination reaction.2. Narrate the background knowledge and characteristics of antigen

25、-antibody interactions.3. Learn about concepts of the agglutination reactions, TITER; precipitation reaction.4. Expound the principle and difference of immunodiffusion and immunoelectrophoresis.5. The principle of test tube agglutination reactions; indirect agglutination inhibition test;counter immu

26、noelectrophoresis第一页教学重点与难点重点：1.Blood type characterization and its importance2.The operation and principle of the immunodiffusion and immunoelectrophoresis3.The operation of tube agglutination reaction 难点：1. The mechanism of antibody-antigen reactions: precipitation,agglutination,coagulation.备 注启发提

27、问1What is blood type? 2What are the concepts of Antigen,antibody,specificity.3. What is the difference between immunodiffusion and immunoelectrophoresis.外语要求1. General immunological terms and experiment methodology description in English.2. Blackboard writing in English.教学手段1. Blackboard description

28、 and multiple media2. Expound the characteristics of antibody-antigen reaction3. Connecting with clinical cases and examples4. Questions5. Showing the picture and cartoon参考资料无注：教案按授课次数填写，每次授课均应填写一份。重复班授课可不另填写教案。生物分子实验教案周 次第 8 周， 第 5 次课 2006年 4 月13 日备 注章 节名 称The Extraction, Separation and Purificatio

29、n of AKP授 课方 式理论课（）；实践课（）；实习（）教学时数6 hours教学目的及要求1.Knowing well the importance of enzyme in medicine andbiology2.Learning enzyme separation and purification,master the principles and operation of organic solvent precipitation of AKP3.Mastering the enzyme protein concentration determination and the sp

30、ecific activity of enzyme4.Mastering the methods of enzyme activity determination教学 主 要内容时间分配1. The importance of enzymes, the nature of enzyme the physical and chemical characteristics of proteins, how to obtain highly active enzyme solution from biological sample.2. The methods of preotein separat

31、ion and purification.3. The principles of the organic solvents to precipitate proteins,the measurement of the specific activity of enzymes.4. The determination of protein concentration of purification sample,quantitative analysis methods of proteins.6. The basic techniques of transfering microlitre

32、liquid and the spectrophotometer.7. University men complete their experiments themselves10min10min5min10min10min3 hrs第一页教学重点与难点重点及难点：1.The principles of the organic solvents to precipitate proteins 2.The measurement of the specific activity of enzymes3.The importance of enzymes, the nature of enzyme

33、 the physical and chemical characteristics of proteins, how to obtain highly active enzyme solution from biological sample.4. The determination of protein concentration of purification sample,quantitative analysis methods of proteins.备 注启发提问1. What is the specific activity of enzyme2. How many metho

34、ds do you know to determinate the enzyme concentration?3. Do you know the principle of the organic solvents to precipitate proteins?外语要求1. General immunological terms and experiment methodology description in English.2. Blackboard writing in English教学手段1. Teaching manifestation,discussed with studen

35、ts.2. Analysis of result reports3. Asking questions4. Power point slides show参考资料无注：教案按授课次数填写，每次授课均应填写一份。重复班授课可不另填写教案。生物分子实验教案周 次第 9 周， 第 6 次课 2006年 4 月20日备 注章 节名 称Determination of Inorganic Phosphate in Serum授 课方 式理论课（）；实践课（）；实习（）教学时数6 hours教学目的及要求1. Determinate the concentration of inorganic phosp

36、hate in serum2. Master the operation of spectrophotometer教学 主 要内容时间分配1. How to use the spectrophotometer2. The principle of determination of inorganic phosphate in serum3. Filtration and calculation in this experiment第一页教学重点与难点重点及难点：1. How to use the spectrophotometer and calculate the concentration

37、 of inorganic phosphate in serum.2. Instructor monitors the students to operate the spectrophotometer and give a score.备 注启发提问1. How to understand the significance of inorganic phosphate in serum.2. What do you know about the reaction mechanism?外语要求1. Narrate common concepts and principles in Englis

38、h.2. Pronounce well and truly,and expound clearly and reposefully3. Thought is legible,and university men understand easily.教学手段1. Brief introduction of exam2. Explain the significance of inorganic phosphate in serum3. Operation by group参考资料无注：教案按授课次数填写，每次授课均应填写一份。重复班授课可不另填写教案。生物分子实验教案周 次第 9 周， 第 6

39、次课 2006年 4 月17日备 注章 节名 称The Characteristics of AKP and Properties of Enzymatic Dynamics授 课方 式理论课（）；实践课（）；实习（）教学时数6教学目的及要求1. Understanding the influence of pH on enzymatic activity2. Understanding influence of temperature on enzymatic activity3. Learning the determination of the Km of AKP, the relati

40、on between enzymatic reaction of V0 and S, Michaelis-Menten Equation4. Knowing the influence of inhibitor on enzyme reaction,three kinds of enzymatic inhibitions5. Knowing the characteristics of AKP and properties of enzymatic dynamics6. Knowing significance and concept of Michaelis constant(Km)7. K

41、nowing the concepts of enzyme inhibition and activity.8. What is enzyme kinetics and how does it provide information about the behavior of enzymes?教学 主 要内容时间分配1. Narrate the enzyme in medical Biochemistry and Molecular Biology.Present the essentiality and fundamentality of enzyme in the process of m

42、etabolism of organism.2. Narrate the some properties of enzymes and the principles of enzyme catalytic reaction, expounding how enzyme structure affect enzyme activity and how are enzyme named and classified.3. Narrate what is enzyme kinetics and how does it provide information about the behavior of

43、 enzymes.Expounding enzyme inhibition and activity.Narrate the influence of pH,temperature,substrate concentration,inhibitor et al. on enzyme activity.4. Introduce the experimental operation procedure and various experimental items.5. Let university men do experiment all by themselves and tutor some

44、 important operations of some experimental items.1510151515270第一页教学重点与难点重点及难点：1. Narrate what is enzyme kinetics and how does it provide information about the behavior of enzymes.Expounding enzyme inhibition and activity.Narrate the influence of pH,temperature,substrate concentration,inhibitor et al. on enzyme activity.2. Introduce the experimental operation procedure and various experimental items.备 注启发提问1.How do enzymes work?2.How do