SCI结果写作技巧.doc

《SCI结果写作技巧.doc》由会员分享，可在线阅读，更多相关《SCI结果写作技巧.doc（10页珍藏版）》请在淘文阁 - 分享文档赚钱的网站上搜索。

1、一、 结果小标题1. MEK inhibitors decrease constitutive ERK activity and cause NSCLC cell cycle arrest in G0/G12. Flow cytometry assays3. Effect of Znf179 knockdown on the cell cycle profile and cell cycle regulatory proteins. 4. Flow cytometric analysis of cell cycle distribution5. Effect of LY2940 02, PD9

2、8059 and rapamycin , on the growth of gag-I R/IGFR- RIE, and v-Src-RI E cells in monolayer6. Effects of ONOO -on morphological changes in FaDu cells7. Effects of curcumin on morphological changes in FaDu cells8. Effects of ONOO -on cell viability in FaDu cells9. Curcumin inhibits cell viability of F

3、aDu cells10. MEK1 inhibition suppresses ERK1/2 activation and inhibits myeloma cell proliferation11. Curcumin inhibits the migration and invasion ability of FaDu cells12. Targeted NOX4 knockdown with a specific siRNA blocks RMF-EG stimulus on MCF-7 migration13. EGFR-MSC demonstrated enhanced migrato

4、ry responses toward tumor-conditioned media, which was partially dependent on EGF and EGFR 14. Effects of curcumin on mRNA expressions of metastasis-related genes in FaDu cells15. Effects of ONOO -on mRNA expression of PDCD4 in FaDu cells 16. Downregulation of T bRII in non-small-cell lung cancers (

5、NSCLC) 17. KLF8 Promotes Cell Proliferation and Invasion, Inhibits Apoptosis, and Induces EMT In Vitro18.二、 结果开头句评估.效果，运用.方法，在.药物浓度以及在.时间作用于.细胞1. In order to assess the cytotoxic effect of curcumin on the human hypopharyngeal cancercells, FaDu cells were cultured with curcumin at final concentration

6、s of 10, 20, 40, 60, 80, and 100 M for 24 h, 48 h, and 72 h, then, MTT assay was carried out respectively.2. Morphological changes of FaDu cells were examined by acridine orange cytochemistry staining at 48 h after treatment with curcumin at 0, 20, 40, 60, and 80 M, respectively.3. The FaDu cell cyc

7、le distribution was analyzed by MultiCycle software to investigate the effect of curcumin on the cell cycle.4. The anti-metastatic activity of curcumin was evaluated using the Transwell assay.5. Western-blot analyses were performed to estimate the levels of caspase-9, caspase-3, Bcl-2 and Bax in FaD

8、u cells in response to curcumin (Fig. 5 A).6. As curcumin inhibited FaDu cells migration and invasion, we further investigated its effect on the metastasis-related genes.7. SGNs were exposed to ONOO(100mM) for 24 h with or without curcumin pretreatment.8. To delineate描绘 the signaling pathway of mito

9、chondria-related apoptosis, we investigated the effect of ONOO on the activation of Apaf-1, Caspase-9 and Caspase-3 genes which are involved in the mitochondrion-dependent apoptosis pathway.9. Flow cytometry assay with Annexin V/PI double staining showed that the apoptotic rate increased to 50.273.2

10、6% after treating with 100mM ONOO for 24 h (Fig. 4B)10. To examine the role of Znf179 in the cell cycle during neuronal differentiation, P19 cells were infected with negative control and Znf179 shRNA viruses. 11. We evaluated cell cycle parameters after PD98059 modulation in four cases: three cases

11、had high ERK activity, one case was ERK negative. 12. To study the possible role of NOX4 in the RMF-EG-mediated stimulus on MCF-7 migration, we used a targeted knockdown by siRNA approach to downregulate the enzyme expression. 13. In order to test our hypothesis that EGFR transfection enhances migra

12、tion of MSCs toward tumor cells, we employed in vitro two-chamber migration assays. First, we tested migratory responses of primary MSCs and EGFR-MSC toward GL261- or B16-conditioned media (Fig 2a). 14. RT-PCR analyses of the mRNA levels of PDCD4 were performed to estimate the effect of ONOO -on the

13、 expression of PDCD4 gene (Fig.4 A). 15. To verify whether TbRII level is downregulated in human lung tumours, we analysed for TbRII expression by RT -PCR using RNA samples from 46 lung tumour specimens (20 squamous cell carcinoma, 19 adeno carcinoma, and seven large cell carcinoma) (Figure 1A). 16.

14、 Since we observed reduced TbRII mRNA expression in NSCLC, we tested the expression of T b RII protein in lysates made from tumour specimens by Western blot analysis. 17. The 1D Ca 2 mRNA level during heart development was carried out by real-time RT-PCR using total RNA isolated from fetal, neonatal

15、, 4- to 6-wk-old, and 6- to 8-mo-old rat atrial tissue using 18S rRNA as an internal control. 18. To investigate the effect of KLF8 in EMT, the expression of EMT markers were assessed.19. As telomerase activity is frequently proposed to be associated with the malignant phenotype, we attempted to det

16、ermine whether a correlation exists between ionizing radiation-induced cell death and telomerase activity.20. good Cells collected at different times postirradiation were examined for cell viability and telomerase activity.21. Since cells in an actively growing tumor are distributed in different cyc

17、le stages, we also attempted to determine whether the telomerase activity in exponentially growing cells is affected by ionizing radiation. We first evaluated cell cycle phase distributions in growing HeLa cells by flow cytometry.22. In view of the correlation between the expression status of hTERT

18、and telomerase activity三、 结果表达：1. 主系表结构：结果做主语主语the trend, the proportions, the percentages, the levels, the expressions, cell viability, the rate, the activity, 系表/谓语1）was significantly observed，2）升高increased to 50.273.26%，were gradually increased，were increased significantly ，was significantly high

19、er，was amplified in glomeruli， 3）降低were reduced，were reduced to73.41 5.84 %，was decreased obviously， was markedly reduced to，were unable to generate a migratory stimulus on MCF-7 cells， 程度副词用于有显著性统计学差异的词significantly， markedly，remarkably；用于形态学表现有差异的词obviously，apparently；其他gradually逐渐地；mildly轻微地, gre

20、atly, appreciably可感觉到地； 1) As shown in Figure 1, a trend of decreasing viability with increasing curcumin concentration and expending treatment-time was significantly observed in FaDu cells, indicating that curcumin inhibited the cell viability in a dose- and time-dependent manner.2) FaDu cells were

21、 treated with 0, 20, and 40 M curcumin for 48 h respectively, as shown in Figure 3, the proportions of FaDu cells in S phase were gradually increased (from 14.23 2.17 % to 23.10 2.33 %, 35.13 3.35 %), meanwhile, the percentages of cells at G0/G1 phase were gradually decreased (from 67.03 3.37 % to 5

22、7.87 4.60 %, 46.10 2.98 %,).3) The levels of migration were reduced to73.41 5.84 % and 58.54 7.76 % of the control (fresh medium alone) level at 10 and 20 M curcumin, respectively (Fig. 4 A, C).4) The protein expressions of cleaved caspase-9 (37 kDa), cleaved caspase-3 (17 kDa) and Bax were increase

23、d significantly in FaDu cells treated with curcumin 20 M and 40 M compared to that of control (Fig. 5 B), while, the expression of Bcl-2 gene was decreased obviously (Fig. 5 B), as well as the decrease of ratio of Bcl-2/Bax (data not shown).5) mRNA expression levels of pro-metastasis genes MMP-2 and

24、 MMP-9 were reduced, whereas, Ecadherin, an anti-metastasis gene, was increased significantly by curcumin at concentrations of 10 M and 20 M, respectively (Fig. 6)6) Cell viability was markedly reduced to approximately 59.573.02% after exposure to 100mM ONOO for 24 h compared with the control group

25、(Fig. 2A,B).7) Flow cytometry assay with Annexin V/PI double staining showed that the apoptotic rate increased to 50.273.26% after treating with 100mM ONOO for 24 h (Fig. 4B), while, the rates of control group and curcumin-pretreated group were 4.361.47% and 7.310.99% respectively (Fig. 4A,C). Stati

26、stic analyses revealed that the apoptotic rate was significantly higher in ONOO group compared with that in control group (Fig. 4D,p0.001), and that the apoptotic rate in the curcumin-pretreated group declined remarkably in comparison with that in ONOO group (Fig. 4D,p0.001).8) The SOD activity of t

27、he ONOO -treated group was 36.783.62% of the control group. In SGNs pretreated with curcumin (15mM, 12 h), the level of SOD activity was 86.193.13% of control group.9) The mRNA expression of Apaf-1, Caspase-9, Caspase-3 was increased significantly after exposure to ONOO 100mM for 24 h as evidenced b

28、y RT-PCR (Fig. 7). Moreover, the mRNA expression of anti-apoptotic gene Bcl-2 was decreased while the Bax gene expression was increased.10) As Figure 3A shows, RMF-EG cells transiently transfected with siNOX4 were unable to generate a migratory stimulus on MCF-7 cells. 11) The T b RII expression was

29、 found to be decreased in 80% of squamous cell carcinoma, 42% adenocarcinoma, and 72% large cell carcinoma12) The PCR product for Hip1 was amplified in glomeruli, whereas no expression was detected in the rest of the kidney lacking glomeruli (Figure 1). 13) The reaction intensity of the immunocytoch

30、emistry was in accordance with qRTPCR and Western blot analyses14) We examined 2 hallmarks of EMT: E-cadherin was down-regulated, and Vimentin was up-regulated in HCC cell lines with high KLF8-expression by qRT-PCR analysis.15) Down-regulation of mesenchymal markers (N-cadherin, vimentin, and fibron

31、ectin) and upregulation of the epithelial marker (E-cadherin) were observed by RT-PCR after KLF8 siRNA (Figure 2F).16) Good The survival of different cell types after exposure to graded doses of radiation (137Cesium gamma rays at 1.1 Gy/min) as determined by clonogenic assay is shown in Fig. 1.17) G

32、ood A strong correlation was detected between telomerase activity and cell viability in HeLa cells treated with ionizing radiation.18) Good A similar pattern of decrease in telomerase activity (Fig. 6) and cell viability (data not shown) with an increase in radiation dose treatment was observed in t

33、he colorectal carcinoma (RKO) cell line.19) Telomerase activity is detectable in most human tumor tissue and in tumor-derived cell lines, but not in normal cells adjacent to tumors or mixed with tumor-derived cell lines (29).20)2. 主谓宾结构：药物做主语主语药物名称curcumin，treatment, pretreatment with, ONOO exposure

34、, the level, Curcumin pretreatment, exposure,主语表达形式curcumin in the range of，treatment with 10 and 20 M curcumin for 24 h，Pretreatment of SGNs with curcumin (5, 10, 15mM)，the pretreatment with curcumin 15mM for 12 h，Curcumin pretreatment (15mM, 12 h)，ONOO exposure，exposure of ONOO (100mM) to SGNs for

35、 24 h，谓语1）降低inhibited，produced a significant reduction，lowered the levels of GSH to 45.157.03%，curcumin pretreatment effectively eliminated除去/消除 the ONOO -induced changes，attenuated减弱，decreased the elevation of MDA level, diminished STK33 protein expression；declined remarkably in，resulted in the red

36、uction of, had a mild inhibitory effect on, displayed a significantly greater resistance toward inhibition of colony, had no appreciable effect on, abolish the increase in G0/G1-phase cells caused by RA treatment, has a role in inhibiting cell cycle progression, we observed significant inhibition of

37、 cell cycle progression, prevented cell cycle progression in XG-6,2）升高elevate, ，resulted in the elevation增高 of， reached at the peak，increased, significantly induced the protein expression of，raised this level to 116.471.75% of control, promotes neuronal differentiation, B16 melanoma-conditioned medi

38、a also enhanced the migration of EGFR-MSC, 程度副词用于有显著性统计学差异的词significantly， markedly，remarkably；用于形态学表现有差异的词obviously，apparently；其他gradually逐渐地；mildly轻微地, greatly, appreciably可感觉到地；1) 转移实验As illustrated in Figure 4, curcumin in the range of 0-20 M inhibited the migration of FaDu cells in a dose-depen

39、dent manner.2) 侵袭实验 Similarly to its effects in the anti-migration assay, treatment with 10 and 20 M curcumin for 24 h produced a significant reduction in the number of invasive cells compared to the control group, the invasion index were 71.18 3.87 %, 49.53 7.83 % respectively (Fig. 4 B,C).3) Pretr

40、eatment of SGNs with curcumin (5, 10, 15mM) could protect against the excitotoxicity elicited引起 by ONOO in a dose-dependent manner and neuroprotection reached at the peak with the concentration of 15mM (97.642.04% of control). However, doses of curcumin higher than 15mM (20, 25, 30 and 50mM) were to

41、xic to cells (Fig. 2A)4) As shown in Fig. 5, ONOO exposure lowered the levels of GSH to 45.157.03% of the control levels, while, the pretreatment with curcumin 15mM for 12 h completely eliminated消除 the ONOO induced changes in GSH level and raised this level to 116.471.75% of control.5) The level of

42、MDA increased significantly in ONOO treated group as compared with control group (207.3624.49% of control). Curcumin pretreatment (15mM, 12 h) decreased the elevation of MDA level compared with ONOO-treated group (85.043.68% of control).6) In this experiment, exposure of ONOO (100mM) to SGNs for 24

43、h resulted in the elevation增高 of cytochrome cin cytosol, and, correspondingly, the reduction of cytochromecin mitochondria. Conversely, pretreatment with curcumin 15mM for 12 h markedly reversed逆转 these consequences (Fig. 6).7) RNA表达 On the other hand, curcumin pretreatment effectively eliminated除去/

44、消除 the ONOO -induced changes in mRNA expressions of Apaf-1, Caspase-9, Caspase-3, Bcl-2 and Bax, respectively (Fig. 7)8) 蛋白表达 Further analysis of protein expression showed that ONOO significantly induced the protein expression of cleaved-Caspase-9 (37 kDa), cleaved-Caspase-3 (17 kDa) and Bax, meanwh

45、ile, inhibited the expression of Bcl-2. On the contrary, pretreatment with curcumin 15mM for 12 h decreased the levels of cleaved-Caspase-9, cleaved-Caspase-3 and Bax while increased the levels of Bcl-2 obviously (Fig. 8), as well as the decrease of ratio of Bcl-2/Bax (data not shown).9) 细胞活性 As evi

46、denced by MTT assay, ONOO -treatment resulted in the reduction of cell viability of the FaDu cells in a concentration-dependent manner (Fig.1). 10) 集落实验PD 98059 at the concentration up to 25 mM had no significant inhibitory effect on the focus formation of all three oncogene transformed cells ( Figu

47、re 8a). It did not inhibit the colony forming ability of gag-I R and gag-IGF R cells either, but had a mild inhibitory effect on that of the v-Src- RIE cells (Figure 9a). LY29400 2 inhibited both colony and focus formation of gag-I R and gag-I GFR transformed RIE cells in a dosage dependent manner (

48、Fi gures 8b an d 9b ). Interestingly, v-Src transformed cells displayed a significantly greater resistance toward inhibition of colony and especially focus forming ability by LY29 4002 (Figures 8b and 9b).11) 集落实验Rapamycin also had a significant effect on colony and focus formation of the gag-I R/IG

49、FR -RIE cells but had no appreciable effect on v-Src- RIE cells in either the colony or the focus forming ability (Figures 8c and 9 c).12) 细胞周期As expected, G0/G1-phase cells in RA-treated P19 cells had increased compared with non-treated cells (Figures 8a and b), in agreement with RA-induced cell cycle arrest and neuronal differentiation in P