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各种缓冲液的配制方法大全.pdf

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1、磷酸氢二钠柠檬酸缓冲液Na2HPO4 分子量=14.98，0.2mol/L 溶液为 28.40 克/升。Na2HPO4 2H2O 分子量=178.05，0.2mol/L 溶液含 35.01 克/升。C4H2O7 H2O 分子量=210.14，0.1mol/L 溶液为 21.01 克/升20%盐酸溶液如何配置：取浓盐酸（质量百分比浓度为36.5%）一个体积（如 100 毫升），加入到 96.5毫升水中就可以了。36.5%*100*1.17=20%*mm=213.5(克)所需水的质量为;213.5-117=96.5克，也就是 96.5毫升水。PH 0.2mol/L Na2H

2、PO4（毫升）0.1mol/L 柠檬酸（毫升）pH 0.2mol/L Na2HPO4（毫升）0.1mol/L 柠檬酸（毫升）2.2 2.4 2.6 2.8 3.0 3.2 3.4 3.6 3.8 4.0 4.2 4.4 4.6 4.8 5.0 0.40 1.24 2.18 3.17 4.11 4.94 5.70 6.44 7.10 7.71 8.28 8.82 9.35 9.86 10.30 10.60 18.76 17.82 16.83 15.89 15.06 14.30 13.56 12.90 12.29 11.72 11.18 10.65 10.14 9.70 5.2 5.4 5.6 5

3、.8 6.0 6.2 6.4 6.6 6.8 7.0 7.2 7.4 7.6 7.8 8.0 10.72 11.15 11.60 12.09 12.63 13.22 13.85 14.55 15.45 16.47 17.39 18.17 18.73 19.15 19.45 9.28 8.85 8.40 7.91 7.37 6.78 6.15 5.45 4.55 3.53 2.61 1.83 1.27 0.85 0.55 磷酸盐缓冲液配制方法（PB）磷酸盐缓冲液（Phosphate Buffer,PB）试剂：NaH2PO4 2H2O Na2HPO4 12H2O 配制方法：配制时，常先配制0.2m

4、ol/L的 NaH2PO4和 0.2mol/L的 Na2HPO4，两者按一定比例混合即成 0.2mol/L的磷酸盐缓冲液（PB），根据需要可配制不同浓度的PB 和 PBS。（1）0.2mol/L的 NaH2PO4；称取 NaH2PO4.2H2O 31.2g(或 NaH2PO4 H2O 27.6g)加重蒸水至 1000ml溶解。（2）0.2mol/L的 Na2HPO4：称取 Na2HPO4.12H2O 71.632g（或 Na2HPO4 7H2O 53.6g或 Na2HPO4 2H2O 35.6g）加重蒸水至1000ml溶解。（3）0.2mol/L pH7.4的 PB 的配制：取19ml 0.2

5、mol/L的 NaH2PO4和 81ml 0.2mol/L的Na2HPO4 12H2O,充分混合即为0.2mol/L的 PB（pH 约为 7.4 7.5）。若 pH 偏高或偏低，可通过改变二者的比例来加以调整，室温保存即可。0.2mol/L磷酸盐缓冲液（pH5.7 8.0）pH 0.2mol/L NaH2PO4(ml)0.2mol/L Na2HPO4(ml)5.7 93.5 6.5 5.8 92.0 8.0 5.9 90.0 10.0 6.0 87.7 12.3 6.1 85.0 15.0 6.2 81.5 18.5 6.3 77.5 22.5 6.4 73.5 26.5 6.5 68.5 3

6、1.5 6.6 62.5 37.5 6.7 56.5 43.5 文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9

7、 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7

8、T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9

9、 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7

10、T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9

11、 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7

12、T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z36.8 51.0 49.0 6.9 45.0 55.0 7.0 39.0 61.0 7.1 33.0 67.0 7.2 28.0 72.0 7.3 23.0 67.0 7.4 19.0 81.0 7.5 16.0 84.0 7.6 13.0 87.0 7.7 10.5 89.5 7.8 8.5

13、91.5 7.9 7.0 93.0 8.0 5.3 94.7 0.05mol/L磷酸盐缓冲液(pH7.0)如何配制？文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档

14、编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8

15、W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档

16、编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8

17、W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档

18、编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8

19、W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档

20、编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8

21、W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档

22、编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8

23、W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档

24、编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8

25、W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档

26、编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8

27、W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档

28、编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8

29、W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档

30、编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8

31、W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档

32、编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3PH3-10 缓冲溶液的配制方法1磷酸氢二钠柠檬酸缓冲液pH 0.2mol/LNa2HPO4（毫升）0.1mol/L 柠檬酸（毫升）pH 0.2mol/LNa2HPO4（毫升）0.1mol/L 柠檬酸（毫升）2.2 2.4 2.6 2.8 3.0 3.2 3.4 3.6 3.8 4.0 4.2 4.4 0.40 1.24 2.18 3.17 4.11 4.94

33、5.70 6.44 7.10 7.71 8.28 8.82 10.60 18.76 17.82 16.83 15.89 15.06 14.30 13.56 12.90 12.29 11.72 11.18 5.2 5.4 5.6 5.8 6.0 6.2 6.4 6.6 6.8 7.0 7.2 7.4 10.72 11.15 11.60 12.09 12.63 13.22 13.85 14.55 15.45 16.47 17.39 18.17 9.28 8.85 8.40 7.91 7.37 6.78 6.15 5.45 4.55 3.53 2.61 1.83 文档编码:CG7T3R3Z5G8 H

34、Y7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5

35、Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 H

36、Y7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5

37、Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 H

38、Y7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5

39、Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 H

40、Y7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z34.6 4.8 5.0 9.35 9.86 10.30 10.65 10.14 9.70 7.6 7.8 8.0 18.73 19.15 19.45 1.27 0.85 0.55 Na2HPO4分子量=14.98，0.2 mol/L 溶液为 28.40 克/升。Na2HPO42H2O 分子量=178.05，0.2 mol/L 溶液含 35.01 克/升。C4H2O7H2O 分子量=210.14

41、，0.1 mol/L 溶液为 21.01 克/升。2柠檬酸氢氧化钠-盐酸缓冲液pH 钠离子浓度（mol/L）柠檬酸（克）C6H8O7H2O 氢氧化钠(克)NaOH 97%盐酸（毫升）HCl（浓）最终体积（升）2.2 3.1 3.3 4.3 5.3 5.8 6.5 0.20 0.20 0.20 0.20 0.35 0.45 0.38 210 210 210 210 245 285 266 84 83 83 83 144 186 156 160 116 106 45 68 105 126 10 10 10 10 10 10 10 使用时可以每升中加入1 克克酚，若最后 pH 值有变化，再用少量

42、50%氢氧化钠溶液或浓盐酸调节，冰箱保存。3 甘氨酸盐酸缓冲液（0.05mol/L）X 毫升 0.2 mol/L 甘氨酸+Y 毫升 0.2 mol/L HCI，再加水稀释至200 毫升pH X Y pH X Y 2.0 2.4 2.6 2.8 50 50 50 50 44.0 32.4 24.2 16.8 3.0 3.2 3.4 3.6 50 50 50 50 11.4 8.2 6.4 5.0 甘氨酸分子量=75.07，0.2 mol/L 甘氨酸溶液含15.01 克/升。4邻苯二甲酸盐酸缓冲液（0.05 mol/L）X 毫升 0.2 mol/L 邻苯二甲酸氢钾+0.2 mol/L HCl，再加

43、水稀释到20 毫升pH(20 )X Y pH(20)X Y 2.2 2.4 2.6 2.8 3.0 5 5 5 5 5 4.070 3.960 3.295 2.642 2.022 3.2 3.4 3.6 3.8 5 5 5 5 1.470 0.990 0.597 0.263 邻苯二甲酸氢钾分子量=204.23，0.2 mol/L 邻苯二甲酸氢溶液含40.85 克/升5柠檬酸柠檬酸钠缓冲液（0.1 mol/L）pH 0.1mol/L 柠檬酸（毫升）0.1 mol/L 柠檬酸钠（毫升）pH 0.1mol/L 柠檬酸（毫升）0.1mol/L 柠檬酸钠（毫升）文档编码:CG7T3R3Z5G8 HY7F

44、8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文

45、档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F

46、8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文

47、档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F

48、8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文

49、档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F

50、8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z3文档编码:CG7T3R3Z5G8 HY7F8W6O7N9 ZM8L9E9T5Z33.0 3.2 3.4 3.6 3.8 4.0 4.2 4.4 4.6 4.8 18.6 17.2 16.0 14.9 14.0 13.1 12.3 11.4 10.3 9.2 1.4 2.8 4.0 5.1 6.0 6.9 7.7 8.6 9.7 10.8 5.0 5.2 5.4 5.6 5.8 6.0 6.2 6.4 6.6 8.2 7.3 6.4 5.5 4.7 3.8 2.8 2.0 1.4

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