复习题2基因重组与基因工程.pdf

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1、1 复习题2 基因重组与基因工程一、A 型题1.实验室内常用的连接外源性DNA 和载体 DNA 的酶是：（A）ADNA 连接酶BDNA 聚合酶CDNA 聚合酶DDNA 聚合酶E反转录酶2.用来鉴定 DNA 的技术是：（B）ANorthern 印迹BSouthern印迹CWestern印迹 D亲和层析E离子交换层析3.下列那项不是重组DNA 技术中常用的工具酶：（D）A限制性核酸内切酶BDNA 连接酶CDNA 聚合酶 DRNA 聚合酶E反转录酶4.关于限制性核酸内切酶的叙述，下列哪项是错误的？（E）A限制性核酸内切酶分为三类，用于基因工程的为酶B限制性核酸内切酶切割后可产生粘性末端或平端C识别的

2、核苷酸序列个数可以是6 个或 8 个D.识别的序列一般具有回文结构E识别的 DN A 为单链5.关于基因工程的叙述，下列哪项是错误的？（B）2 A基因工程也称基因克隆 B只有质粒 DNA 可作为载体C重组体 DNA 转化或转染宿主细胞 D需获得目的基因E需对重组 DN A 进行纯化、筛选6.有关质粒的叙述，下列哪项是错误的？（E）A小型环状双链DNA 分子 B可小到 23Kb 大到数百个 Kb C能在宿主细胞中独立自主地进行复制 D常含有耐药基因 E只有一种限制性核酸内切酶切口7下列哪项不是重组DN A 的连接方式？（C）A粘性末端与粘性末端的连接B平端与平端的连接 C.粘性末端与平端的连接D

3、人工接头连接 E同聚物加尾连接8 DNA 克隆不包括下列哪项步骤？（E）A选择一个适合的载体B限制性核酸内切酶在特异位点裂解质粒和目的基因C用连接酶连接载体DNA 和目的 DNA，形成重组体文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:

4、CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J

5、1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:

6、CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J

7、1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:

8、CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J

9、1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H53 D用载体的相应抗生素抗性筛选含重组体的细菌E重组体用融合法导入细胞9基因重组是指 DNA 分子的：（A）A共价连接B氢键连接C离子键连接D交换E退火10在分子生物学中

10、，重组DNA 又称为：（D）A.酶工程 B.蛋白质工程 C.细胞工程 D.基因工程 E.DNA 工程11基因工程中，不常用到的酶是（E）A限制性核酸内切酶 BDNA 聚合酶CDNA 连接酶 D逆转录酶EDNA 解酶12能识别 DNA 的特异序列，并在识别位点或其周围切割双链DNA 的一类酶称为：（B）A.DNA 内切酶 B限制性内切核酸酶C非限制性内切核酸酶 D限制性外切核酸酶E非限制性外切核酸酶13cDNA 是指：（A）A在体外经反转录合成的与RNA 互补的 DNA B在体外经反转录合成的与DNA 互补的 DNA C在体外经反转录合成的与RNA 互补的 RNA D在体内经反转录合成的与RNA

11、 互补的 RNA 文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H

12、5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT

13、3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H

14、5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT

15、3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H

16、5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT

17、3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H54 E在体内经反转录合成的与RNA 互补的 DNA 14质粒的分子结构是：（A）A环状双链 DNA B环状单链 DNA C环状单链 RNA D线状双链 DNA E线状单链 DNA 15聚合酶链式反应常常缩写为：（E）APRC BPER CPDR DBCR EPCR 16用于 PCR 反应的酶是：（E）ADNA 连接酶B碱性磷酸酶C逆转录酶

18、D限制性内切核酸ETaq DNA 聚合酶17基因工程中使目的基因与载体拼接的酶是：（C）ADNA 聚合酶BRNA 聚合酶CDNA 连接酶 DRNA 连接酶E限制性核酸内切酶18 互补筛选属于：（C）A抗药性标志筛选B酶联免疫筛选 C标志补救筛选 D原位杂交筛选E免疫化学筛选19确切地讲，cDNA 文库包含：（E）A一个物种的全部基因信息 B一个物种的全部RNA 信息 C一个生物体组织或细胞的全部基因信息文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5

19、HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L

20、7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5

21、HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L

22、7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5

23、HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L

24、7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5

25、HC8N2C1J1T7 ZT3K1Q4L7H55 D一个生物体组织或细胞的全部RNA 信息E一个生物体组织或细胞所表达mRNA 信息20下烈描述最能确切表达质粒DNA 作为克隆载体特性的是：（D）A小型环状双链DNA 分子B携带有某些耐药基因C 在细胞分裂时恒定地传递给子代细胞D 具有自我复制能力E获得目的基因21.基因工程中常用限制性内切酶的识别序列，正确的说法是：（E）A.聚胞苷酸B.聚腺苷酸C.6 或 8 个任意核苷酸D.4 或 6 个任意核苷酸E 具有回文结构22基因工程的基本过程不包含：（E）A.DNA 重组体的形成及转化B.限制酶的切割C.载体及目的基因的分离D.重组体的筛选与鉴定

26、E.重组体的序列分析文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9

27、H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 Z

28、T3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9

29、H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 Z

30、T3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9

31、H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 Z

32、T3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H56 23.有关理想质粒载体的特征，正确的是：（B）A.其复制受宿主控制B.含有多种限制性内切酶的单一切点C含有同一限制酶的多个切点D为线性单链 DNAE不含耐药基因24.下列那个物质一般不用作基因工程的载体：（C）A噬菌体B质粒C大肠杆菌基因组D反转录病毒 DNAE人工酵母染色体25.基因工程的操作程序可简单的概括为：（E）A将重组体导入

33、宿主细胞并筛选B限制酶的应用C将载体和目的基因接合成重组体D目的基因和载体的分离提纯与鉴定E分、切、接、转、筛26.基因表达的多级调控不包括：（A）文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1

34、T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:C

35、V6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1

36、T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:C

37、V6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1

38、T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:C

39、V6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H57 ADNA 复制B转录起始C转录后加工D.翻译起始E.翻译后加工27.下列基因工程中的目的基因的来源，最不常用的是：（C）A.人工合成 DNAB从 mRNA 合成 cDNAC.从真核生物染色体DNA 中直接分离

40、D从基因文库中获取E从细菌基因组 DNA 中直接分离28.型限制性内切酶是：（B）A有内切酶和甲基化酶活性B.仅有内切酶活性，甲基化酶活性由另外一种酶提供C有外切酶和甲基化酶活性D限制性识别非甲基化的核苷酸序列E仅有外切酶活性，甲基化酶活性由另外一种酶提供29.基因工程操作应用的许多载体质粒都有抗生素的抗性基因，这是为了便于：（D）文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B

41、5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K

42、1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B

43、5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K

44、1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B

45、5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K

46、1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H58 A外源基因插入质粒 B

47、.宿主菌的生物繁殖 C质粒的转化 D带有目的基因的宿主菌的筛选 E目的基因的表达30.有关目的基因与载体连接的叙述错误的是：（E）A.可以同一限制酶切割产生粘性末端连接B.平末端切口可采用“尾接法”C平末端切口可直接连接D均需 DNA 连接酶参与E不同限制型内切酶切割不能连接1基因工程的单元操作顺序是（A ）酶切，连接，转化，筛选，验证酶切，转化，连接，筛选，验证连接，转化，筛选，验证，酶切验证，酶切，连接，筛选，转化酶切，连接，筛选，转化，验证2下列五个 DNA 片段中含有回文结构的是（D、E ）GAAACTGCTTTGAC GAAACTGGAAACTG GAAACTGGTCAAAG GAA

48、ACTGCAGTTTC GAAACTGCAGAAAG 3T 4-DNA 连接酶是通过形成磷酸二酯键将两段 DNA 片段连接在一起，其底物的关键基团是（D ）2-OH 和 5-P 2-OH 和 3-P 文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5

49、文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8

50、N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5文档编码:CV6X9H5B5X5 HC8N2C1J1T7 ZT3K1Q4L7H5