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1、实验九生长素类物质对水稻根芽生长的不同影响1实验原理实验原理生长素是第一个被发现的植物激素,对植物生长有很大影响,其作用体现浓度效应:对某器官而言,低浓度表现促进效应;高浓度起抑制作用,不同的植物或同一植物的不同器官,对生长素的浓度反应都有差异。因此,生长素类物质对器官生长有一个最佳促进浓度。不同器官对生长素的敏感性不同,因此最佳促进浓度不同,促进生长的程度亦不同。一般根对生长素的敏感程度要比芽大得多,所以,根对生长素所要求的最适浓度要比芽低得多。萘乙酸(NAA)是一类人工合成的生长素类物质,对根、芽生长的不同影响与生长素一致。本实验将观测萘乙酸的不同浓度对水稻在根、芽生长上的不同影响。11/
2、11/20222实验材料和器材实验材料和器材器材:恒温箱,9cm培养皿10套,9cm圆形滤纸10张,10 mL移液管2支、l mL移液管1支,镊子1把,直尺1把 试剂:10mg/L NAA 实验材料水稻种子11/11/20223实验步骤实验步骤种子培养:取大小一致的试材种子100粒,每皿10粒,沿培养皿四周均匀地播开加盖后放入25恒温箱中黑暗培养3天。测定:取出各号皿中种子,量取各粒种子的根数、根长,芽长。记入下表并计算平均数。配制NAA浓度梯度:给10套培养皿分别编号,在号皿中加9mL10mg/LNAA溶液;在号皿中加1mL10mg/LNAA,再加9mL蒸馏水,配成1mg/LNAA;从号皿中
3、吸取1mL于号皿中,加9mL蒸馏水,配成0.1mg/LNAA液;依次配制梯度浓度,使至号皿中NAA浓度分别为;10、1、10-1、10-2、10-3、10-4、10-5、10-6、10-7mg/L,从最后的号皿中吸去1mL溶液。号皿中加入9mL蒸馏水作对照。在每个皿中加入一张圆形滤纸。11/11/20224实验报告实验报告1、为何要从9号皿中吸去1 mL溶液?2、在该实验中,如果培养皿的盖子盖得不严,有水分挥发,对实验结果有何影响?3、在该实验中使用移液管时,应注意些什么 11/11/202257IaMdPgSkVnZq$t*x-A1D5G8KbNeQiTlWo#r%v(y+B3E6H9LcO
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