中药学专业毕业论文（Graduation thesis of Chinese pharmacy）.doc

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1、中药学专业毕业论文Graduation thesis of Chinese pharmacyThe 1.1 longqirougan capsules of longqirougan capsules for the preparation of Xinyang Hospital of Traditional Chinese Medicine hospital, composed of Astragalus, Salvia miltiorrhiza, Radix Codonopsis, radix rehmanniae, Radix Glehniae, angelica, white peon

2、y, earthworm, vinegar Biejia, polygonatum, Schisandra chinensis, Ganoderma lucidum, peach kernel, safflower, fried pangolin total of 15 traditional Chinese medicine prepared. Indications: chronic viral hepatitis, liver fibrosis, cirrhosis and other diseases. It has the effects of Supplementing Qi an

3、d nourishing Yin, promoting blood circulation and softening the liver. In the square earthworm has the strong peculiar smell, the pangolin belongs to the rare medicinal material, uses the powder to be used as medicine. Because the capsule has the characteristics of covering the bad odor of the medic

4、ine and the large drug loading, the capsule 4 is chosen. 1.2 analysis of prescription with Huangqi and earthworm as monarch drug in prescription, Salvia miltiorrhiza, Radix Angelicae sinensis, radix rehmanniae, Radix Codonopsis, Radix Glehniae as adjuvant drug. The spleen lung with Huangqi into the

5、square, pness, Sheng Qing Yang, Yi lung, real fur, with uprighting; with the earthworm by the liver and spleen, Xifeng Tongluo Zhijing, to open the blood block, two were as monarch drug. Salvia, angelica and blood, blood, and blood stasis, Tongluo without injury of blood; rehmannia, Radix Glehniae a

6、nd nourishing yin and nourishing yin and Tonifying Qi to blood; dangshen; five were for official medicine. Take the two ministers, five officials, and the odd system of italy. Schisandra chinensis, Radix Paeoniae Alba common vinegar auxiliary gentleman medicine or grabbing Yin Yang, with soft liver;

7、 turtle, Huang Jing, Ganoderma lucidum common help medicine nourishing liver yin. A total of five adjuvants. Peach kernel, safflower, promoting blood circulation and removing stasis, pangolin swelling and expelling pus, and the three medicines are combined to make up the meridian, all of which are u

8、sed to make medicine. This is BUZHONGYIQITANG (spleen and stomach) and Yang also five soup (correction of the compound method. One is to invigorate Qi, the other is to invigorate the circulation of blood and remove stasis, and the two are used to achieve the effect of Supplementing Qi, nourishing Yi

9、n, promoting blood circulation and softening the liver (5). 1.3 、 pharmacological analysis of Chinese medicine, Astragalus contains polysaccharides and saponins, can enhance non-specific immunity, including increased WBC and neutrophils, M phagocytosis. The extract of earthworm orally has the anti-t

10、umor effect on mice S180 and H22, and can increase the activation of 5-Fu receptor, thereby enhancing the activity of immune cells. The main content of tanshinone A and tanshinone A in Salvia miltiorrhiza and conclusiondss can inhibit myocardial contractility, reduce sinus node automaticity, can pro

11、mote repair and regeneration of various tissues of liver, bone and skin, which promote the repair and regeneration of liver tissue is significantly 3. Therefore, tanshinone A is also the important and key components of drugs. 1.4 summary this article explores the establishment and optimization of HP

12、LC method for the determination of longqirougan capsules of tanshinone A content method, to evaluate the quality of the preparation and provide experimental basis to improve the quality of the compound preparation. The American company Agilent, the 2. 2.1. test instrument 2.1.1 Agilent-1200 liquid c

13、hromatography Agilent-1200 detector, Agilent workstation; Waters-1525 liquid chromatograph (Waters, Waters2489 oven Breeze workstation); precese dual range electronic balance America Kayseri staples company; super pure water machine (genotype 185OD Chongqing Moore water equipment Co., Ltd.) ultrasou

14、nd; (Ningbo scientz Biotechnology Co. Ltd). 2.1.2 reagent of tanshinone A control (product specifications: 20mg / 110766-200518, (China; pure chromatography, pharmaceutical and biological products by methanol) American Agilent company); longqirougan capsules (Xinyang Hospital of Traditional Chinese

15、Medicine preparation room, specifications: 60 grains / bottle, batch number: 20210410, 20210512, 20210823); (water in Xinyang City Institute of traditional Chinese medicine laboratory water machine, water level); other reagents were analytically pure. The results of 2.2. method and 2.2.1 standard so

16、lution preparation of tanshinone A, control 10.6mg 25ml volumetric flask, accurately weighed, constant volume with methanol. The 1ml pipette was used to remove the disposed reference solution, and the 25ml volumetric flask was added to the fixed volume of methanol. The reference solution concentrati

17、on is worthy of preparation of 16.96ug / ml.2.2.2 sample solution and negative samples of the drug in 5 copies, each 3.2g, precision said. The same model 250ml, with a conical flask, and each with 25ml methanol. Precise setting.Ultrasonic extraction of 30min. Placed at room temperature, weighed accu

18、rately and used methanol to make up for the amount lost. After filtration, the filtrate is obtained. To prepare the negative sample without Salvia miltiorrhiza, the negative sample solution was prepared according to the preparation method of the sample. Table 1 the sampling volume of ultrasonic ultr

19、asound after complement after No. 1 experiment 3.1873 116.3690 116.3269 116.36422 112.2992 1123.2871 112.29313, experiment 3.1770 experiment 3.1933 94.3075 94.2945 94.30214 104.3507 104.3348 104.35205, experiment 3.2188 experiment 3.1842 88.1713 88.1548 88.17482.2.3 chromatographic column: Agilent C

20、18 column (250mm * 4.6mm5um); mobile phase: methanol: water (20:80) as mobile phase; flow rate: 1.0mlmin-1; detection wavelength: 270nm; column temperature: 25 degrees. The number of theoretical plates should not be less than 2000 calculated by Tanshinone A peak . The degree of separation of 1.5; ot

21、her ingredients in longqirougan capsules on Determination of tanshinone A content had no effect . 1. control samples 2. samples 3. missing 2.2.4 linear relationship in the control of precision from the configured A, tanshinone referencesolution 1ul, 3ul, 5ul, 7ul, 9ul, HPLC, according to the determi

22、nation of the peak area integral value of the vertical axis (Y), the amount of sample (UG) as the abscissa (X) standard curve. The results showed that tanshinone and A showed a good linear relationship in the range of ml / 16.96152.64ug. The regression equation: Y5759589X-101938R20.99969. sample vol

23、ume sample volume (g) retention area time peak area 1 by 15.511 2573 75.463 183679 82.315 15.511 383743 15.537 edged slightly edged up 91.797 15.504 580916 97.889 781003 91.91 900000 5759589.24336 y by 15.496 X - 101938.21441 R2 800000 0.99969 700000 600000 500000 400000 300000 200000 100000 0 peak

24、area of 0 0.0200 0.0400 0.0600 0.0800 0.1000 0.1200 0.1400 0.1600 0.1800 00000000 00 -100000.00000 injection volume of test precision from the middle of the 2.5 2.2.5 control injection HPLC, repeated sampling 6 times, each sample of 5ul. The record of tanshinone A peak area, the results of tanshinon

25、e ARSD1.69%n52.2.6 reproducibility from the same samples, according to the 2.3 under 5 preparation methods for sample solution, respectively, determination of the peak area, the average content of tanshinone A was calculated and 0.081 mg of g-1, RSD2.67% (N5). 2.2.7 stability test take the same test

26、 solution, respectively in 0, 3, 6, 9h according to the above chromatographic conditions test, tanshinone A records peak area integral value RSD3%; tested the basic stability of solution in 9h. 2.2.8 sample recovery test, precise determination of the content of the sample has been determined (16.96

27、UG / ml), a total of 5 copies, each 3.2g, according to 2.2 methods to prepare the sample solution. Respectively adding precision of tanshinone A control products reserve liquid 1ml injection. The calculation of tanshinone A content, and calculate the recovery rate was 101.32RSD2.8%n5. The content of

28、 2.2.9 samples from 3 batches of longqirougan capsules samples, according to the above test solution preparation method, and the determination of tanshinone A content according to the chromatographic conditions. Results 0.081 mg g-13. discussed 3.1 detection wavelength selection after Agilent-1200 3

29、D map analysis, found that the absorption peak at 270nm275nm and the absorption coefficient are larger, taking into account the longqirougan capsules ingredients are complex, detection of tanshinone A and prone to interference, so the detection wavelength was 270nm. 3.2 selection of chromatographic

30、conditions with methanol: water (25:75) as mobile phase 1, but the peak tailing of tanshinone A,Used for quantitative analysis inaccuracies. After testing, the mobile phase is methanol: water (20:80). The peak was obviously improved. The factors affecting the peak is also with other factors (such as

31、 pH), still need further study. 3.3 selection methods of 3.3.1 drug pretreatment from longqirougan capsules of 4 copies, each 3.2g, precision said. Place 250ml in a conical flask. Number is back 1, back 2, super 1, over 2. Each bottle is accurately added with methanol, and 50ml is called fixed weigh

32、t. 3.3.2 extraction of the sample solution, 30min reflux extraction was performed on the back 1, and 45min reflux extraction was performed on the back 2. Ultra 30min was performed by ultrasound extraction of 45min over 2, ultrasonic extraction by 1, and 1 by ultrasound. 3.3.3 screening of the test s

33、amples, the above four bottles of test materials were placed at room temperature, precise calibration, to make up for the amount of loss, filtration. The liquid was tested with HPLC. When 30min is found, it is extracted completely. Ultrasonic extraction is better than reflux extraction. 4. reference

34、 1 National Pharmacopoeia Committee. Pharmacopoeia of Peoples Republic of China. A s. Beijing China medicine science and Technology Press 2021:2 Zhang Deqin. Analysis of traditional Chinese medicine preparation technology. Beijing: press of traditional Chinese medicine Chinese et al. 3. Wu Qing and

35、pharmacology of traditional Chinese medicine. Beijing: Science Press 4. Joan Chen of Chinese medicine preparation technology. Beijing: China Agricultural University press 5 king. It. Xue Jianguo. Formulaeology. Beijing: Science Press 6 Ling rule. Pharmacy. Shanghai: Shanghai science and technology p

36、ress 7 Zhang Shantang, Chen Xiangqing, Li Qin Tang, the determination of pigment and different M. content of psoralen supplement Chinese pharmacist, psoralen from different Psoralea corylifolia.HPLC method 2021, 3:377 3785. thanks in March, this spring, after spring I will graduate. The graduation t

37、hesis also received a satisfactory conclusion for my college life painting. The writing of this thesis benefits from my practice teacher, Liu Ju. I would like to express my deep appreciation to her. During the course of his writing, he also benefited from the Institute of drug control. Thank you for

38、 your guidance. In the school period also was educated at. Teacher, thank you for being here. This paper was also received during writing. Thank you for your help. During the period of writing, I have received the help of many other teachers, colleagues and classmates. I would like to express my gratitude to you.