自动核酸提取仪原理PCR(新).ppt

《自动核酸提取仪原理PCR(新).ppt》由会员分享，可在线阅读，更多相关《自动核酸提取仪原理PCR(新).ppt（19页珍藏版）》请在淘文阁 - 分享文档赚钱的网站上搜索。

1、全自动核酸提取仪基本原理n n1 磁笔式n n 国外：Chemagenn n 国内：厦门百维信，厦门欧达科，博日n n2 吸管式n n 国外：Roche，Qiagen，生物梅里埃Chemagen 核酸提取仪原理示意图Chemagen主要产品n nchemagic Prepito n nchemagic MSM I chemagic Prepitochemagic Prepiton n1 12 samples per run 1 12 samples per run n nintegrated buffer dispensing integrated buffer dispensing n nr

2、evolutionary resuspension technology revolutionary resuspension technology n nstandard plastic devices standard plastic devices n nchemagic Kits and protocols for a huge variety of chemagic Kits and protocols for a huge variety of sample materials sample materials n nquantitative yield,highest purit

3、y quantitative yield,highest purity n nsample sizes up to 600 l sample sizes up to 600 l n nbar code reading availablebar code reading availablechemagic MSM Ichemagic MSM In nSample volumes from 1 l to 10 ml processable Sample volumes from 1 l to 10 ml processable n nHigh speed,15 min.for 96 low vol

4、ume blood High speed,15 min.for 96 low volume blood samples samples n nLow to high throughput(4,000 samples/d)Low to high throughput(4,000 samples/d)n n96 rod head for parallel separating and 96 rod head for parallel separating and resuspending in 96 well plates resuspending in 96 well plates n n12

5、rod head for parallel separating and 12 rod head for parallel separating and resuspending in 15 ml or 50 ml Falcon tubes resuspending in 15 ml or 50 ml Falcon tubes Fig.1 Schematic illustration of the chemagic Magnetic Separation Module I for semi-automated extraction of viral nucleic acids from up

6、to 12 minipools in one batch.(a)Separation head containing 12 metal rods.(b)Plastic disposables.(c)Tubes containing samples.(d)and(e)Tubes containing wash buffers.(f)Tubes containing elution buffer.其他生产商产品n n厦门百维信 Lab-Aid 820n n厦门欧达科 MP-120 n n博日 Gene-pureGene-pure 工作示意图工作示意图Qiagen BioRobot M96 Workstation BioRobot M48 workstation BioRobot EZ1 RocheMagNA Pure LC 1.0，2.0BioRobot M48 Workstation Qiagen M48 原理示意图Roche MagNA Pure LC原理示意图