分子生物学技术在污水处理微生物检测中的应用.ppt

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1、分子生物学技术在污水处理微生物检测中分子生物学技术在污水处理微生物检测中的应用的应用环境科学学报第25 卷第9 期2005 年9 月评论：xie5彭绪运Main content中文摘要英文摘要 标题 关键词分析 句 句 句修改致谢绿色表示需要修改的，蓝色表示修改后的。分子生物学技术在污水处理微生物检测中的应用分子生物学技术在污水处理微生物检测中的应用摘要摘要：对现代分子生物学技术在污水生物处理系统微生物检测和群落分析中的应用进行了系统总结，重点对应用较多的PCR技术、FISH技术进行了介绍并对其优缺点进行了探讨。通过对生物处理系统中微生物检测和生态学研究，可以分析确定系统中功能微生物的数量,群

2、体结构和活性，从而实现构建人工强化生态系统，提高废水生物处理效率的目的。分析认为，以DNA序列和相关的结构基因为基础的分子生物学技术已经在污水生物处理系统的种群分析方面得到了广泛和成功的应用，而且必将会对深入认识和优化污水生物处理过程，加快新工艺的开发和应用以及强化处理工艺的过程控制方面起到更大的作用。关键词关键词:污水生物处理;分子生物学技术;微生物群落分析The applications of molecular biological tools to microbial ecosystem analyses in wastewater treatment processAbstract:

3、The use of molecular biological tools to microorganism detections and community analyses in the biological wastewater treatment process had been discussed,and the major points were put on the more frequently used techniques such as polymerase chain reaction(PCR)and fluorescence in situ hybridization

4、(FISH).The advantages and disadvantages of each technique were analyzed.Through the detections and analyses,the information of the amounts,the community structures and the activities of the microorganism in the system could be abtained,so these informations can be used to build enhanced ecosystem an

5、d to strengthen the efficiency of the existed system.It is believed that,the molecular biological tools based on the DNA sequences and the relevant structure genes have been successfully used in community analyses in the biological wastewater treatment process,and will surely help to get a deeper in

6、sight into and optimize the biological treatment process.Furthermore,it will promote the developments and applications of novel techniques,and play an even more important role in the process controlling of the biological wastewater treatments.Keywords:biological wastewater treatment;molecular biolog

7、ical techniques;analyses of microorganism community标题分子生物学技术在污水处理微生物检测中的应用The applications of molecular biological tools to microbial ecosystem analyses in wastewater treatment process分析评论：1.标题：中英文不一致2.tools应改为 techniques（以下同）3.中文标题没有表现出有ecosystem应该修改为：The applications of molecular biological techni

8、ques to microbial analyses in wastewater treatment process关键词关键词:污水生物处理;分子生物学技术;微生物群落分析Keywords:biological wastewater treatment;molecular tools;analyses of microorganism community分析：作为分子生物学技术一般常用：molecular biological techniques修改为Keywords:biological wastewater treatment;molecular biological techniqu

9、es;analyses of microorganism community句（本文）对现代分子生物学技术在污水生物处理系统微生物检测和群落分析中的应用进行了系统总结，重点对应用较多的PCR技术、FISH技术进行了介绍并对其优缺点进行了探讨。The use of molecular biological tools to microorganism detections and community analyses in the biological wastewater treatment process had been discussed,and the major points wer

10、e put on the more frequently used techniques such as polymerase chain reaction(PCR)and fluorescence in situ hybridization(FISH).The advantages and disadvantages of each technique were analyzed.analysis加上本文句修改“现代”，“系统总结”没有翻译出来，“探讨”翻译欠妥修改为：This study is systematic reviewed that the application of mode

11、rn molecular biological techniques to microorganism detections and community analyses in the biological wastewater treatment process,and the major points were put on the more frequently used techniques such as polymerase chain reaction(PCR)and fluorescence in situ hybridization(FISH).The advantages

12、and disadvantages of each technique were discussed.句通过对生物处理系统中微生物检测和生态学研究，可以分析确定系统中功能微生物的数量,群体结构和活性，从而实现构建人工强化生态系统，提高废水生物处理效率的目的。Through the detections and analyses,the information of the amounts,the community structures and the activities of the microorganism in the system could be abtained,so thes

13、e informations can be used to build enhanced ecosystem and to strengthen the efficiency of the existed system.analysis句修改1.analysis是分析的意思，而“研究”一般不用analysis.2.“功能”没有翻译出来。3.abtained应该改为obtained综合修改为：Through the detections and researches,the information of the amounts,the community structures and the a

14、ctivities of the functional microorganism in the system could be obtained,so these informations can be used to construct strengthened ecosystem and to enhance the efficiency of the biological wastewater treatment process.句分析认为，以DNA序列和相关的结构基因为基础的分子生物学技术已经在污水生物处理系统的种群分析方面得到了广泛和成功的应用，而且必将会对深入认识和优化污水生物处

15、理过程，加快新工艺的开发和应用以及强化处理工艺的过程控制方面起到更大的作用。It is believed that,the molecular biological tools based on the DNA sequences and the relevant structure genes have been successfully used in community analyses in the biological wastewater treatment process,and will surely help to get a deeper insight into and

16、optimize the biological treatment process.Furthermore,it will promote the developments and applications of novel techniques,and play an even more important role in the process controlling of the biological wastewater treatments.analysis句修改Through analysis，it is believed that,the molecular biological

17、 techniques based on the DNA sequences and the relevant structure genes have been widely and successfully used in community analyses in the biological wastewater treatment process,and will surely help to get a deeper insight into and optimize the biological treatment process.Furthermore,it will prom

18、ote the developments and applications of novel techniques,and play an even more important role in the process controlling of the biological wastewater treatments.修改 This study is systematic reviewed that the application of modern molecular biological techniques to microorganism detections and commun

19、ity analyses in the biological wastewater treatment process,and the major points were put on the more frequently used techniques such as polymerase chain reaction(PCR)and fluorescence in situ hybridization(FISH).The advantages and disadvantages of each technique were discussed.Through the detections

20、 and researches,the information of the amounts,the community structures and the activities of the functional microorganism in the system could be obtained,so these informations can be used to construct strengthened ecosystem and to enhance the efficiency of the biological wastewater treatment proces

21、s.Through analysis，it is believed that,the molecular biological techniques based on the DNA sequences and the relevant structure genes have been widely and successfully used in community analyses in the biological wastewater treatment process,and will surely help to get a deeper insight into and optimize the biological treatment process.Furthermore,it will promote the developments and applications of novel techniques,and play an even more important role in the process controlling of the biological wastewater treatments.Thank you