方穗山羊草Rubisco活性及小亚基基因克隆和功能分析_郭.pdf

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1、植物学报?的?,势?一?注曰的丑角明血口?阮比口方穗山羊草?活性及小亚基基因克隆和功能分析郭绍东“吴光耀吴相枉“?北京大学生命科学学院,北京?猖?摘要以方穗山羊草?月吧放娜?为母本,普通小麦?阮?喇?为父本杂交,并以普 通小麦为父本回交?代获得 的核质杂种小麦 为实验材 料,测定了父母本及其核质杂种小麦?业石?的梭化活性和加氧活性。同时以方穗山羊草幼苗叶片为材料构建了库容量为?护画的碑?文库,并以水稻?三?部分片段为探针筛选该?文库,获得了方穗山羊草成?的?克隆?。核昔 酸序列分析表明该?全长?饰。比较以上三种材 料 中凡山?活性和小 亚基氨基 酸的差异,推测小亚基上第?、?、?和?位氨基酸残

2、基可能对酶的功能起着重要的作用。关键词方穗山羊草,核质杂种小麦,?二。小亚基?习?侧匕?竹?工?肛?义汀污?刃?伙认?面?“,叭?耀一?!兀?一?关“?公泌伙卯?匀仑?,八石月?穷,?巧吨?仪阳?加。,?讨?,?州及塑?,了减忿?一?加?面?场?舍?杭?占,“朋飞招?早?板?刃?,?即?,?娜?川巧?护内?州?叮“。?”。贺?叮?祀?衍?卿?,?到?奴?习?甲】?】?巧坤?一?,?一?幻xyl*/o x yg ell巴犯(nlbis c o)伙朋A.阿aw it ht ha tf rol llT.a e sta n dt h ei rn ude o祀yt叩la s而chy-b r idwhea

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42、lda52 5bpo fco din g。-t r a nsitp eptidea nd128欲苗n oa cid此sidu es ofI n atu此详P tideo f气丁氏洲伟肠3 21 3 4铭1 8 21CGCGGCCGCTCTCCTCCTAGCAT弋3CI、AIATACClA人丁、(Jl人t一、GCC、丫G安c e二e A乏cc苹。A丫G。念e T乏。:,乏。G之e 二e T乏c G革eG之Tc二T二午ee分。G乳片c 女。T乏e工e姿c。名e片ce二ce不e、乱e昙c c毖e:乏c、女cG人5LGG GC;C】A G CC TCG CC、乱c荟e吞e NGGRlAACGGT GG

43、A AGG ATC、兰。T乞e八丫。1 13 9”邵3 31 3 5撰e义。o羊G认尸PIETGGCCCAl,CGAGGGGTATC灸。资e T早e ETLSCAG ACC CTG TCCT支C毓65LPPL5TEG231 TTG CCA CCG:T CA G CA C GG A GG G Ce牛e LKQVDYCl,TAAGCAGGTCGACTACe李。黑81了RSK认,VPCLE279ATCCGCTCCAAGTGGGTGCCCTGCCTCGAGT票T RKVGF96AGAAAGGTTGGGTIC 326791FREHNV3 27 ATCTTC CGT GAA CAC AACGTAT之e P

44、GYYDGCCT GGGTACTACGATGGCe兰G T互C;、VTMWKLPMT GGAC AA T GT GGAA AC T CC 叮A T GT车e GCPGGATGC CCC。之e e二e 工e e足G;翌;里;。草G 李e e呈e G支GG华e e支。EVRKEG A GG T CA G GA A GG A GT支c c异C贫e e忍e T夏T;lCR1IGTGCC凌:A T CA TCG G AT李ee吴e、义eA丫。c兰Te分eo苹。e另e T孚e o革e 昙e;盆;T车e 李e姿c T乏c A灸GPPGGEECCA CCG GGC TGCGAG GAGT乏e漏A灸G AGCC

45、 TAA;ACA C成刃,CAC丁 C AC GAC A GG C C下A TA T AA A AG T GC C A了丁GC A GT T丁T GT6 1招C A AC T CT G AC A TT G CT T TG C GT T TT C CT T CT C CA T TT A TC T TT C TT A TT T GT T CC 叮 AA G AA T AT G TG T AT G TC C AT G TT C AT G TA C CA A CA T GG C T6 6 27 106 6 3 5 6 7 6 1 57 1 1C G AG A AA G CA T GC T CC T A

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