转导骨桥蛋白短发夹状RNA预防动脉粥样硬化模型兔血管成.doc

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1、转导骨桥蛋白短发夹状RNA预防动脉粥样硬化模型兔血管成形后的再狭窄孙玉梅1，2，张金盈1，闫继锋2，袁 斌2，杨鹏伟2，李 文2，于运福21郑州大学第一附属医院心内科，河南省郑州市 450000；2河南省胸科医院心血管内科，河南省郑州市 450000Transduction of osteopontin short hairpin RNA in prevention of restenosis after angioplasty in a rabbit model of atherosclerosisSun Yu-mei1, 2, Zhang Jin-ying1, Yan Ji-feng2,

2、Yuan Bin2, Yang Peng-wei2, Li Wen2, Yu Yun-fu21Department of Cardiology, First Affiliated Hospital, Zhengzhou University, Zhengzhou 450000, Henan Province, China; 2Department of Cardiology, Henan Provincial Chest Hospital, Zhengzhou 450000, Henan Province, China摘要背景：血管成形后再狭窄严重限制了经皮冠状动脉介入治疗的应用和远期疗效。平

3、滑肌细胞的表型转变，增殖是血管成形后再狭窄的重要机制。目的：探讨利用球囊在体转导骨桥蛋白短发夹状RNA，通过抑制实验性动脉粥样硬化模型兔损伤血管部位骨桥蛋白的表达，预防血管成形后再狭窄。方法：构建动脉粥样硬化模型兔20只，随机等分成空质粒组和OPN-shRNA质粒组，分别利用球囊在腹主动脉导入OPN-shRNA质粒和空载体。结果与结论：2组兔球囊扩张后血管平滑肌层出现特异性绿色荧光，且随转染后时间的延长荧光强度逐渐降低，与空质粒组相比，OPN-shRNA质粒组兔扩张动脉的管腔面积明显增加，而斑块负荷明显减小。提示在动脉粥样硬化兔模型局部血管利用球囊导管能成功地转导OPN-shRNA质粒，被扩张

4、血管的再狭窄程度减轻，血栓负荷减轻。这对于预防模型兔血管成形后再狭窄的发生具有十分重要的意义。中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程全文链接：关键词：动物模型；组织工程；RNA干扰；短发卡样RNA；骨桥蛋白；再狭窄；基因治疗；动脉粥样硬化；血管成形；平滑肌细胞； Abstract：BACKGROUND:Restenosis after angioplasty severely limited the application and long-period therapeutic effects of perc

5、utaneous coronary intervention. Changes in smooth muscle cell phenotype and their proliferation are important mechanisms of restenosis after angioplasty.OBJECTIVE: To use balloon in vivo transduction of osteopontin short hairpin RNA (OPN-shRNA), to inhibit osteopontin expression at the injured blood

6、 vessels of a rabbit model of experimental atherosclerosis, and to prevent restenosis after angioplasty.METHODS: A total of 20 rabbit models of atherosclerosis were established and randomly equally assigned to empty plasmid group and OPN-shRNA plasmid group. The plasmid recombinant OPN-shRNA and emp

7、ty plasmid were transferred to the ventral aorta by balloon.RESULTS AND CONCLUSION: After balloon dilatation, specific green fluorescence was detected in the layer of vascular smooth muscle in the two groups. Moreover, with prolonged time of transfection, fluorescence intensity gradually decreased.

8、Compared with the empty plasmid group, the expanded artery lumen area obviously increased in the OPN-shRNA plasmid group, and plaque burden evidently reduced. Results indicated that balloon catheter used in regional blood vessels in rabbit models of atherosclerosis could successfully transduce OPN-s

9、hRNA plasmid. The restenosis of the expanded blood vessels lessened, and thrombus burden relieved. It is of great importance to prevent the occurrence of restenosis after angioplasty in rabbit models.中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程全文链接：Key words：atherosclerosis;coronar

10、y artery disease;RNA interference;osteopontin;myocytes, smooth muscle;收稿日期:2014-02-07 中图分类号:R318基金资助:湖北省科技攻关课题(2006AA301C18)通讯作者:张金盈，博士，主任医师，博士生导师，郑州大学第一附属医院心内科，河南省郑州市 450000作者简介: 孙玉梅，女，1978年生，湖北省咸宁市人，汉族，硕士，主治医师，主要从事经皮冠状动脉介入治疗术后再狭窄的研究。引用本文:孙玉梅,张金盈,闫继锋等. 转导骨桥蛋白短发夹状RNA预防动脉粥样硬化模型兔血管成形后的再狭窄J.中国组织工程研究, 2014, 18(18): 2801-2805.Sun Yu-mei,Zhang Jin-ying,Yan Ji-feng et al. Transduction of osteopontin short hairpin RNA in prevention of restenosis after angioplasty in a rabbit model of atherosclerosisJ. Chinese Journal of Tissue Engineering Research, 2014, 18(18): 2801-2805.