红叶杨和竹柳的抗寒能力评价研究,风景园林硕士论文.docx

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1、红叶杨和竹柳的抗寒能力评价研究,风景园林硕士论文 本篇论文目录导航：【第1部分】 红叶杨和竹柳的抗寒能力评价研究【第2部分】【第3部分】【第4部分】【第5部分】【第6部分】 摘 要 红叶杨和竹柳因其生长迅速、观赏效果极佳，是近年来极具应用前景的优良园林绿化树种，但有关其抗寒性的研究尚未见报道。本文以红叶杨和竹柳为试验材料，研究了低温胁迫对其相对电导率、丙二醛MDA含量、可溶性糖含量、可溶性蛋白质含量以及保卫酶活性的影响，讨论了红叶杨和竹柳的抗寒性生理机制；利用 Logistic方程确定了其低温半致死温度，并结合从属函数法对红叶杨和竹柳的抗寒能力进行综合评价，获得的研究结果如下： 1在低温胁迫处

2、理经过中，随着温度的逐步降低，红叶杨和竹柳的离体枝条和根系的相对电导率均表现出逐步升高的变化规律，但变化幅度存在差异。在-20时，竹柳枝条的相对电导率达 50%以上，红叶杨枝条在-15时相对电导率为 55.83%,而竹柳和红叶杨的根系在-10时相对电导率分别为 49.83%和 53.93%.根据试验数据并结合 Logistic 方程确定了四种供试材料的半致死温度LT50：红叶杨枝条及根系的半致死温度分别为-11.46和-6.24，竹柳枝条及根系的半致死温度分别为-15.83和-10.07。 2随着低温胁迫的逐步下降，四种材料中的丙二醛MDA含量均呈现逐步增加的趋势，竹柳枝条在各低温胁迫条件下丙

3、二醛MDA含量最低且增加幅度最小。根据丙二醛MDA方差数据分析显示：竹柳枝条具有较强的抗寒能力，红叶杨根系的抗寒性最弱。 3随着温度的逐步降低，四种材料的 SOD 酶活性均表现出先升高后降低的趋势。红叶杨枝条及根系从初始阶段到-15时 SOD 酶活性逐步上升，在-15时到达峰值，之后随着低温处理 SOD 酶活性逐步下降；竹柳枝条及根系从初始阶段到-20时 SOD 酶活性逐步升高，上升趋势较红叶杨枝条以及根系较为缓慢，在-20时 SOD酶活性到达峰值，之后酶活性逐步下降。POD 酶活性变化趋势与 SOD 酶活性变化趋势大致类似。 4经低温处理后，红叶杨和竹柳的枝条以及根系的可溶性糖含量均呈现逐步

4、上升的趋势，从试验材料中可溶性糖含量的变化趋势分析得出，抗寒性较强的试验材料可溶性糖含量显着高于抗寒性弱的试验材料。 5在低温处理条件下，各试验材料的蛋白质含量呈先上升后降低的趋势。在一定温度范围内，蛋白质含量升高，红叶杨枝条及根系在-15到达顶峰，然后蛋白质含量逐步降低；竹柳枝条及根系在-20到达顶峰，然后蛋白质含量逐步降低。但抗寒性较差的品种其蛋白质含量明显少于抗寒性较好的品种，讲明蛋白质含量与植物的抗寒性密切相关。 6通过对红叶杨和竹柳枝条及根系相对电导率、超氧化物歧化酶SOD活性、过氧化物酶POD活性、丙二醛MDA含量、可溶性糖含量和可溶性蛋白质含量等 6个指标的分析，并运用模糊概率综

5、合评价方式方法，根据综合评价数据对参试材料的抗寒性进行排序，四种供试材料的抗寒性强弱顺序依次为：竹柳枝条 红叶杨枝条；竹柳根系 红叶杨根系即竹柳 红叶杨。该综合评价结果与枝条恢复生长结果基本一致，讲明利用模糊概率评分法对红叶杨和竹柳进行抗寒性分析评价是可行的。 本文关键词语：红叶杨；竹柳；抗寒性；相对电导率；丙二醛MDA；超氧化物歧化酶SOD；过氧化物酶POD Abstract Populus deltoids cv. Zhonghua hongye and Salicaceae sp. belong to the largesize of the tree, because of its r

6、apid growth and excellent viewing, application themin landscape greening will have obvious effect .But in the process of introduction andCultivation indifferent regions, affected by the low temperature of environment, two kindsof plants often damage and even death phenomenon in winter.In this paper,

7、 take theannualred Populus deltoids cv. Zhonghua hongye and Salicaceae sp. as the testmaterial, they from the Baoding City of the Hebei province,studied the physiologicalcharacteristics under the different and low temperature , and by determining the relativeconductivity, MDA, solublesugar and solub

8、le protein content, protective enzyme andcombined with the trend growth rate of recovery of shoots seven relevant experimentalindex. Study of cold resistance physiological mechanism of Populus deltoids cv. Zhonghua hongye and Salicaceae sp. comprehensive the evaluation combined withmembership functi

9、on method of two kinds of plant cold resistance, through the testingindex data analysis results show that: 1In the low temperature treatment process, gradually decreases with the temperature,the relative conductivity of the body of branches and roots from Populus deltoids cv.Zhonghua hongye and Sali

10、caceae sp. showed gradually increased, but there weredifferences between the amplitude of variation.At -20 , the relative electricconductivity value in the branch of Salicaceae sp. has reached more than50%, the relative electric conductivity value in branches of Populus deltoids cv. Zhonghua at -15

11、has reached 55.83%, and the relative electric conductivity in theroots of Populus deltoids cv. Zhonghua hongye and Salicaceae sp. reached49.83% and 53.93% at -10 。According to the test data and the Logistic equation isdetermined of four kinds of tested materials and semi lethaltemperature LT50： semi

12、 lethal temperature in the branches and roots of Populusdeltoids cv. Zhonghua hongye are -11.46 and -6.24 , semi lethaltemperature in the branches and roots of Salicaceae sp. are -15.83 and -10.07 。 2The measure of MDA in different and low temperature and conditions showedthat: the contents of MDA i

13、n all tests of materials gradually decreases with thetemperature .The MDA in The branch of Salicaceae in the low temperature was thelowest and the smallest increased. The results of variance analysis showed that: in thetested materials, The branch of Salicaceae sp. has strong cold resistance, the ro

14、ot system ofPopulus deltoids cv. Zhonghua hongye has he worst cold resistance. 3According to the analysis of the test data in the low temperature condition of artificial,the results showed that: the activity of SOD in all materials showed the downward.Trends after the first rise.The SOD activities o

15、f the branches and roots in Populus deltoidscv. Zhonghua hongye from the initial stage to -15 increased gradually, and reachedthe peak at -15， SOD activity treatment decreased after low temperature;From the initialstage to -20 ，the SOD activity in the branch and root of Salicaceae sp. graduallyincre

16、ased, the rising trend is relatively slow than the branch and root of Populus deltoidscv. Zhonghua hongye . and SOD enzyme activity reached the peak at -20， then theenzyme activity gradually decreased .The trend of POD activity and SOD activity isbasically the same. According to the result of varian

17、ce analysis showed that the significantdifference between different varieties of SOD and POD enzyme activity. 4The soluble sugar content in different product test materials showed agradual upwardtrend after low temperature treatment, the analysis from the trend of soluble sugar contentof test materi

18、als, soluble sugar content in resistant variety was higher than that in the weakcold resistance varieties. 5Under low temperature condition, the protein content of each test material increasedfirst and then decreased. In a certain temperature range, the increase in protein content,the branches and r

19、oot of Populus deltoids cv. Zhonghua hongye reached the peak at -15， the protein content decreased gradually after the branches and root of Salicaceae sp.reached the peak at -20， the protein content decreased gradually after the tend. But thecold hardiness variety of poor its protein content was sig

20、nificantly less the than that ofvarieties better cold tolerance, cold resistance and plant is closely related to the proteincontent. 6Through the relative conductivity of the branches and roots of Populus deltoidscv. Zhonghua hongye and Salicaceae sp. in the relative conductivity, SOD activity, PODa

21、ctivity, MDA content, soluble sugar content and soluble protein content of 6 indicators. And use the fuzzy comprehensive with evaluation method of probability, sorted based onthe cold resistance of tested materials comprehensive evaluation data, its cold resistance inorder:the branches of Salicaceae

22、 sp. the branches of Populus deltoids cv.Zhonghuahongye the roots of Salicaceae sp. the roots of Populus deltoids cv. Zhonghuahongye Salicaceae sp. Populus deltoids cv. Zhonghua hongye。The comprehensiveevaluation result of Populus deltoids cv. Zhonghua hongye and Salicaceae sp. in coldhardiness and

23、branches to restore growth and field performance is basically the same, byusing fuzzy probability score were evaluated with the feasibility of Populus deltoidscv. Zhonghua hongye . Keywords: Populus deltoids cv. Zhonghua hongye ; Salicaceae sp. ; Electricalconductivity ;Cold resistance;MDA;SOD;POD 目

24、 录 1 引言 1 1.1 红叶杨和竹柳研究进展 1 1.1.1 红叶杨研究进展 1 1.1.2 竹柳研究进展 3 1.2 植物抗寒性研究进展 4 1.2.1 生物膜与抗寒性关系的研究 4 1.2.2 丙二醛MDA含量变化与抗寒性关系的研究 6 1.2.3 可溶性蛋白质含量变化与抗寒性关系的研究 6 1.2.4 可溶性糖含量变化与抗寒性关系的研究 7 1.2.5 保卫酶与抗寒性关系的研究 8 1.2.6 植物恢复生长与抗寒性关系的研究 8 1.3 本研究的目的和意义 9 2 材料与方式方法 10 2.1 试验地大概情况 10 2.2 试验材料和方式方法 10 2.3 指标测定方式方法 10 2

25、.3.1 相对电导率测定方式方法 10 2.3.2 丙二醛MDA含量测定方式方法 11 2.3.3 可溶性蛋白质含量测定方式方法 11 2.3.4 可溶性糖含量测定方式方法 12 2.3.5 超氧化物歧化酶SOD活性测定方式方法 13 2.3.6 过氧化物酶POD活性测定方式方法 13 2.3.7 枝条恢复生长测定方式方法 14 2.4 数据处理方式方法 14 3 结果与分析 15 3.1 低温胁迫对红叶杨和竹柳细胞膜透性的影响 15 3.2 低温胁迫对红叶杨和竹柳丙二醛MDA含量的影响 17 3.3 低温胁迫对红叶杨和竹柳可溶性蛋白质含量的影响 20 3.4 低温胁迫对红叶杨和竹柳可溶性糖含

26、量的影响 21 3.5 低温胁迫对红叶杨和竹柳超氧化物歧化酶SOD活性的影响 23 3.6 低温胁迫对红叶杨和竹柳过氧化物酶POD活性的影响 26 3.7 红叶杨和竹柳的抗寒性从属函数综合评价 28 3.8 红叶杨和竹柳的测定指标和温度的相关性分析 29 3.9 红叶杨和竹柳枝条恢复生长分析 31 4 讨论 33 4.1 测定指标与抗寒性研究的关系 33 4.2 细胞膜与抗寒性研究的关系 33 4.3 质膜过氧化作用与抗寒性研究的关系 34 4.4 细胞内保卫物质与抗寒性研究的关系 34 4.5 保卫酶与抗寒性研究的关系 35 5 结论与建议 36 5.1 结论 36 5.2 建议 37 以下为参考文献 38 致 谢 43