最新布鲁菌属-微生物教学课件PPT课件.ppt

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1、布鲁菌属布鲁菌属-微生物教学课件微生物教学课件第二篇 致病性细菌第24章布鲁菌属Brucella 微生物学教研室 钟照华教授 商庆龙讲师 Department of Microbiology,Harbin Medical UniversityDepartment of Microbiology,Harbin Medical UniversityDepartment of Microbiology,Harbin Medical UniversityDepartment of Microbiology,Harbin Medical UniversityDepartment of Microbiol

2、ogy,Harbin Medical UniversityDepartment of Microbiology,Harbin Medical UniversityDepartment of Microbiology,Harbin Medical University流行环节Department of Microbiology,Harbin Medical University分泌物、乳品分泌物、乳品消化道、呼吸道粘消化道、呼吸道粘膜、皮肤及眼结膜膜、皮肤及眼结膜人人菌血症菌血症16W潜伏期潜伏期（70）发热、出汗、发热、出汗、关节痛和全关节痛和全身乏力身乏力内毒素内毒素进入肝、脾、骨髓进入肝、

3、脾、骨髓（发热消退）（发热消退）繁殖入血繁殖入血菌血症菌血症（体温再次上升）（体温再次上升）波浪热波浪热Department of Microbiology,Harbin Medical University致病性n n致病物质uu内毒素、透明质酸酶、荚膜内毒素、透明质酸酶、荚膜Department of Microbiology,Harbin Medical University所致疾病n n母畜流产uu赤藓醇胎盘、绒毛膜含量高赤藓醇胎盘、绒毛膜含量高Department of Microbiology,Harbin Medical Universityuu波浪热波浪热 Undulant f

4、ever Undulant fever体温逐日上升，达到高热程度后，持续若干时日，再逐渐降至正常，经过数日后又重发作，如此互相交替 Department of Microbiology,Harbin Medical University微生物学检查法n n分离培养与鉴定n n血清学试验uu凝集试验凝集试验uu补体结合试验补体结合试验uu抗球蛋白试验抗球蛋白试验FFIgAIgA不完全抗体干扰试验不完全抗体干扰试验n n皮肤试验uu布鲁菌素（布鲁菌素（brucellinbrucellin）试验）试验Department of Microbiology,Harbin Medical Universi

5、ty防治原则n n严格管理病畜，消灭动物感染n n预防接种uu家蓄家蓄uu易感人群易感人群FF牧区、屠宰场工作人员牧区、屠宰场工作人员n n急性期抗生素治疗uu四环素、青霉素四环素、青霉素Brucella species S u m m a r yS u m m a r yDepartment of Microbiology,Harbin Medical UniversityBiologyn nSmall gram-negative rods without capsulen nObligatory aerobicn nGrows slowly in enriched culture medi

6、a with 10%CO2n nH2S+Department of Microbiology,Harbin Medical UniversityPathogens Human pathogen Animal reservoirs1.1.Brucella melitensis goat,sheep2.2.Brucella abortus cattle3.3.Brucella suis pigDepartment of Microbiology,Harbin Medical UniversityTransmissionn nEnter human body by uuIngestion of In

7、gestion of contaminated milk productscontaminated milk productsuuThrough the Through the skin directly contactskin directly contact in an in an occupational setting such as occupational setting such as abattoirabattoir Department of Microbiology,Harbin Medical UniversityPathogenesisn nBrucella local

8、ized in the reticuloendothelial system(lymph nodes,liver,spleen,and bone marrow)n nCause granulomatosis,abscess,and caseationn nNo exotoxin is produced,so endotoxin is the major virulent factor Department of Microbiology,Harbin Medical UniversityDisease:Brucellosisn nAnimaluuAbortionAbortionn nHuman

9、uuWeakness and fatigueWeakness and fatigueuuEnlarged lymph nodes,liver,and spleenEnlarged lymph nodes,liver,and spleenuuUndulant feverUndulant fever(rising-and-falling fever)(rising-and-falling fever)FFOccurs in a few patientsOccurs in a few patientsDepartment of Microbiology,Harbin Medical Universi

10、tyDiagnosisn nIsolationuuSample:blood,bone marrowSample:blood,bone marrown nSerological examinationuuAgglutination testAgglutination testuuComplement fixation testComplement fixation testuuCoombs test Coombs test n nSkin testDepartment of Microbiology,Harbin Medical UniversityPrevention&Therapyn nPasteurization of milkn nImmunizationuuAnimalsAnimalsuuOccupational high-risk personsOccupational high-risk personsn nSlaughtering of infected animalsn nTreatment with tetracycline and penicillin结束语结束语谢谢大家聆听！谢谢大家聆听！24