《寄生虫实验》PPT课件.ppt
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1、ExperimentIIIExaminationofFecalsamples粪便检查法粪便检查法1.Placeadropofisotonicsalineonaslide.2.Takeasmallpieceoffeceswithatoothpickandstiritintothesaline.3.Addacoverslipornot,andexaminemicroscopically.一、直接涂片法(一、直接涂片法(DirectSmear)二、饱和盐水浮卵法(二、饱和盐水浮卵法(FecalFlotation)Mix several grams(a teaspoonful)of feces wit
2、h the flotationsolutioninaplasticcup.Strain the mixture through a cuprum strainer(60 mesh)intoanotherplasticcup.Allowtheflotationtostandforatleast5minutes.Takeafewdropsofthesurfaceofthesolutionwithcuprumwirelooponamicroscopeslide,examinethemunderthemecroscopewiththe10lens.三、沉淀法(三、沉淀法(Fecal Sedimenta
3、tion)Mixabout100mlofwaterwithabout10goffecesandplaceinaplasticcup.Strainthemixturethroughacuprumstrainer(60mesh)intoanothercup.Allowmixturetositfor10minutesandthendecantthesupernatant.Add more water,mix,and repeat the sedimentation procedure until thesupernatantwillbeveryclear.Stir remaining mixture
4、 and place a few drops on a microscope slide,examinethemunderthemecroscopewiththe10lens.家禽体内的寄生虫卵家禽体内的寄生虫卵1.鸡蛔虫卵鸡蛔虫卵2.鸡异刺线虫卵鸡异刺线虫卵3.类圆线虫卵类圆线虫卵4.孟氏眼线虫卵孟氏眼线虫卵5.旋华首线虫卵旋华首线虫卵6.四棱线虫卵四棱线虫卵7.鹅裂口线虫卵鹅裂口线虫卵8.毛细线虫卵毛细线虫卵9.鸭束首线虫卵鸭束首线虫卵10.比翼线虫卵比翼线虫卵11.卷棘口吸虫卵卷棘口吸虫卵12.嗜眼吸虫卵嗜眼吸虫卵13.前殖吸虫卵前殖吸虫卵14.次睾吸虫卵次睾吸虫卵15.背孔吸虫卵背孔吸
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