硕士生物仪器分析之气相色谱讲解学习.ppt

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1、硕士生物仪器分析之气相色谱ChromatographyIsatechniqueusedtoseparateandidentifythecomponentsofamixture.Worksbyallowingthemoleculespresentinthemixturetodistributethemselvesbetweenastationaryandamobilemedium.Moleculesthatspendmostoftheirtimeinthemobilephasearecarriedalongfaster.IntroductionGasChromatographyHerethemo

2、bilephaseisanunreactivegas(egNitrogen)flowingthroughatube.Andthestationaryphaseisaninvolatileliquid heldonparticlesofasolidsupport.IntroductionGasChromatography(GC)isnowamature analytical tool particularlyusefulintheanalysisofvolatileandthermallystableanalytes.Characteristic 气相色谱可用来分析能能瞬瞬间间气气化化且且不不分

3、分解解的、的、沸点0.1%)GC analysis of fragrances格拉斯格拉斯(Grasse)世界香水之都世界香水之都 提问时间Future Trends in the Separation Sciences1.MiniaturizationComparisonamongGCcolumns(inscale)ComparisonamongHPLCcolumns(inscale)packed columnscapillary columns2.Neither temperature,nor pressure:electrical field GCthecolumntemperature

4、HPLCapressuregradientsolventselectrodrivenelectrophoretictechniques(hybridtechniquesthatuseaspectsofchromatographyaswellasaspectsofelectrophoresis)Future Trends in the Separation Sciences3.Hyphenated systemsLC-LC(suchas:aRP-18column+achiralcolumn)SFE-CESFE-SPE-HRGCLC-GCSPME-GC-MSSFE-SFCFuture Trends

5、 in the Separation Sciences4.The role of the solvent in separation sciencesFuture Trends in the Separation Scienceshttp:/www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-505320030002000055.High speed separation techniques6.Automation of the separation techniques7.Unified chromatographyFuture Tr

6、ends in the Separation SciencesGeneralseparationapproachemployedinatypicalcomplexanalysis1.ExtractionofsolidsamplesExtraction TechniquesSoxhletextractionMicrowaveextractionSonicationandsimilartechniquesPressurizedsolventtechniqueSupercriticalfluidextraction(SFE)Subcriticalwaterextraction(SWE)Acceler

7、atedsolventextraction(ASE)2.ExtractionofliquidsamplesLiquid-liquidextraction(LLE)Solid-phaseextraction(SPE)Solidphasemicroextraction(SPME)Stirbarsorptiveextraction(SBSE)Pressurizedsolventextraction(PSE)SFESWEASEExtraction Techniques3.ExtractionofgaseoussamplesHeadspacetechniquethestaticheadspace(SH)approachesthedynamicheadspace(DH)approaches Headspace-Solidphasemicroextraction(HS-SPME)Extraction Techniques此课件下载可自行编辑修改，仅供参考！此课件下载可自行编辑修改，仅供参考！感谢您的支持，我们努力做得更好！谢谢感谢您的支持，我们努力做得更好！谢谢