环境DNA的英语介绍ppt课件.pptx

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1、在整堂课的教学中，刘教师总是让学生带着问题来学习，而问题的设置具有一定的梯度，由浅入深，所提出的问题也很明确Environmental DNA for wildlife biology and biodiversity monitoring Bai Jie在整堂课的教学中，刘教师总是让学生带着问题来学习，而问题的设置具有一定的梯度，由浅入深，所提出的问题也很明确CONTENTSCONTENTS01Basic Conception02Experimental Method03Practical Application04Future Prospect在整堂课的教学中，刘教师总是让学生带着问题来学

2、习，而问题的设置具有一定的梯度，由浅入深，所提出的问题也很明确Part One在整堂课的教学中，刘教师总是让学生带着问题来学习，而问题的设置具有一定的梯度，由浅入深，所提出的问题也很明确01Basic Conceptionsequencing of the total DNA extracted from a sample containing many different organisms.eDNAOneMetagenomicsThreeAmplificationTworefers to any DNA that is collected from an environmental sam

3、ple rather than directly from an organism,originating in cells from the body or waste products(saliva,urine,faeces)of organismsa fragment of DNA or RNA created by replication events or amplification,either naturally or artificially,through,for example,PCR.在整堂课的教学中，刘教师总是让学生带着问题来学习，而问题的设置具有一定的梯度，由浅入深，

4、所提出的问题也很明确01Basic ConceptionSNLU non-invasive sampling that may be particularly important for threatened or endangered speciesNon-invasiveand identification of target organisms using uniform,reproducible criteria that are accurate over different life stagesUniform sampling of locations that are unsa

5、fe or difficult to access with traditional methodsLow-requirementachieving sensitive detection probabilities for low abundancespeciesSensitive在整堂课的教学中，刘教师总是让学生带着问题来学习，而问题的设置具有一定的梯度，由浅入深，所提出的问题也很明确eDNA historyeDNA historyB BD DA AC CThe American Bullfrog(Lithobates catesbeianus)was successfully ident

6、ified,showing that early detection of invasive species at low densities is possible and has implications for management Fresh waterEnvironmental DNA as a method to assess the diversity of macro-organismal communities was first applied to sediments revealing DNA from extinct and extant mammals,birds

7、and plants,followed by dry cave sediments,Later,it was shown that also DNA from invertebrates suchas insects,as well as fungisedimentsMetabarcoding of eDNA from surface soil has thus efficiently been used as a proxy for plant taxonomic diversity in several different terrestrial ecosystemssoilEnviron

8、mental DNA arose with the idea of obtaining nucleic acids of microbes directly from environmental samples microbial在整堂课的教学中，刘教师总是让学生带着问题来学习，而问题的设置具有一定的梯度，由浅入深，所提出的问题也很明确Part Two在整堂课的教学中，刘教师总是让学生带着问题来学习，而问题的设置具有一定的梯度，由浅入深，所提出的问题也很明确01Sequencing02Application03It is possible to obtain sequence It is po

9、ssible to obtain sequence information from the environment information from the environment without isolating the target specieswithout isolating the target speciessamplingStudies that necessitate rapid or Studies that necessitate rapid or multiple species detection are possible multiple species det

10、ection are possible and ideally suitedand ideally suitedapplicationCombined with 2Combined with 2ndnd Generation Generation Sequencing,thousands or millions of Sequencing,thousands or millions of sequences can be produced sequences can be produced simultaneously to analyze species simultaneously to

11、analyze species diversity.diversity.sequencing02Experimental MethodSampling在整堂课的教学中，刘教师总是让学生带着问题来学习，而问题的设置具有一定的梯度，由浅入深，所提出的问题也很明确Part Three在整堂课的教学中，刘教师总是让学生带着问题来学习，而问题的设置具有一定的梯度，由浅入深，所提出的问题也很明确EndangeredInvasiveExtremelysubterranean2013201403Practical在整堂课的教学中，刘教师总是让学生带着问题来学习，而问题的设置具有一定的梯度，由浅入深，所提出的问

12、题也很明确Practical Application在整堂课的教学中，刘教师总是让学生带着问题来学习，而问题的设置具有一定的梯度，由浅入深，所提出的问题也很明确在整堂课的教学中，刘教师总是让学生带着问题来学习，而问题的设置具有一定的梯度，由浅入深，所提出的问题也很明确Part Four在整堂课的教学中，刘教师总是让学生带着问题来学习，而问题的设置具有一定的梯度，由浅入深，所提出的问题也很明确04Future Prospect Miniaturization has resulted in the development of portable field instruments that ca

13、n amplify,screen,and even sequence eDNA in remote settings,such as Biomeme two3,MinION.In the future,it may be possible to implement mechanical sampling of eDNA,similar to that of oil spill-sampling buoys or military sonobuoys.在整堂课的教学中，刘教师总是让学生带着问题来学习，而问题的设置具有一定的梯度，由浅入深，所提出的问题也很明确future researchThe

14、temporal and spatial distribution of eDNA in different habitats,which gives information as to what part of the biodiversity is monitored in space and time.01The exact sources of eDNA,whether this comes from sloughed epithelial cells,intestinal cells,faeces and urine,etc.,which might vary with life s

15、tages of the target organism and could therefore greatly influence abundance estimates.03More precise links between eDNA concentration and species abundance whether this is measured as total biomass or density of individuals.02Physio-chemical factors influencing eDNA availability and degradation suc

16、h as temperature,pH and salinity.04在整堂课的教学中，刘教师总是让学生带着问题来学习，而问题的设置具有一定的梯度，由浅入深，所提出的问题也很明确summaryeDNA is on the brink of making significant contributions to our understanding of invasive species,community and ecosystem processes underpinning biodiversity and functional diversity,and wildlife and cons

17、ervation biology.在整堂课的教学中，刘教师总是让学生带着问题来学习，而问题的设置具有一定的梯度，由浅入深，所提出的问题也很明确Reference 1.Evans,N.T.and G.A.Lamberti,Freshwater fisheries assessment using environmental DNA:A primer on the method,its potential,and shortcomings as a conservation tool.Fisheries Research,2018.197:p.60-66.2.Rees,H.C.,et al.,RE

18、VIEW:The detection of aquatic animal species using environmental DNA-a review of eDNA as a survey tool in ecology.Journal of Applied Ecology,2014.51(5):p.1450-1459.3.Jerde,C.L.M.A.,“Sight-unseen”detection of rare aquatic species using environmental DNA.Conservation Letters,2011.4(2):p.150-157.4.TABERLET,P.,et al.,Environmental DNA.Molecular Ecology,2012.21(8):p.1789-1793.5.Bohmann,K.,et al.,Environmental DNA for wildlife biology and biodiversity monitoring.Trends in Ecology&Evolution,2014.29(6):p.358-367.在整堂课的教学中，刘教师总是让学生带着问题来学习，而问题的设置具有一定的梯度，由浅入深，所提出的问题也很明确ThanksFor Your Watching