17微生物复习2169.pdf

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1、17 115 I.Heterotroph 2.1 nterfero n(IFN)3.Mutatio n DNA4.Capsule 5.Haustorium 6.Disin fecti on 7.Autotrophs(Auxoautotrophs?)C02 C028.SPF 9.Phage(bacteriophage)10.In herita nee(heredity)17 215 II.Con ti nu ous culture 12.Plasmid DNA13.Col ony 14.Pasteurizatio n（Pasteur dis infection?）100C15.Tran sduc

2、ti on DNA16.poly-3-hydroxybutyrate（PHB）-3-0.2-0.7m17.Respiratory cha in 18.Virule nt bacteriophage 17 315 19.Muta nt strain 20.Growth factors 1._ 17 415 ABCD2._ ABCD3.A.B.C.D.4.ABCD5.ABCD6.G+G-A.B.C.D.7.ABCD8.A.B.C.D.9.Streptomyces A mould B fungi C act inom ycetes D archaea10.ABCD11.E.coliABCD12.A.

3、95%-96%B.70%-75%C.60%-70%D.70%-75%14.A.1B.2C.3D.4 15.13.EMP110A.21ATP B.12ATP D.11ATP 17 515 A.Rickettsia B.E.coli C.Mycoplasma D.Short spiral body of swine dysentery16.ABCD17._ ABCD18.The most effective way of sterilizing a solution is by A.B.C.D.19.A BCD20.PDAABCD21.Bacterial endospores function i

4、n A.B.C.D.22.ABCD23.CO2ATPH(_)A.NH 4+NO2-H2SS0 B.NH 4+NO3-H2SS C.NH 4+NO2-SO4-S0 D.NH 3NO2-H2S S0 24.A.-SH B.-OH C.-CH3 D.-COH 25.ABCD26.A.B.C.D.L27.A.B.C.D.28.17 615 ABCD29.1861A.B.C.D 30.T4A.B.C.D.1.T2.Escherichia coliG+G+TG-3.T4.SalmonellaSalmonellaT5.F1-F0ATPADPPiATPT6.7.plasmid DNAT8.Rhizopus T

5、9.T10.poly-hydroxybutyrete PHB-T11.T12.T13.14.15.16.17.16S rDNAT18.LomasomeT17 715 19.BeggiatoaC02C02T20.T21.F1-F0ATPADPPiATPT22.23.24.25.26.T27.28.T29.100010000100010000T 1000 30.DNA1._ 2.3.4.5._ 6._ RNADNA7.58._ 9.1 ml17 815 300100310._ 11.Gram12.13.14.15.Staphylococcus aureus Saccharomyces cerevi

6、siae 16.BRNADNA17.518.19.20.DNADNA21._ 22.Saccharomyces cerevisiae _ Bacillus thuringiensis _ 23.24._ pBR322DNA25.26._ 27._ 28.DNA429.J30.17 915 17 1015 1.2G+G-123.Envelope Capsid Nucleic acid Qrum:hArkies-17 1115 HibofonwiTiny pvKiM CompoMI d proftiin and R#MA that ar c E proiBi syntfwig.FlagAlhj2d

7、 Xh*d ic1MuU&v Owl bomac hoidi a long roEMng Mmtni Thif rftgvfmBrt pu|ha ttwetil 1ind prawkiM 4.The Yeast Cell Pliasma membrane Vfcuole RffflKfSOflie MllechcndnM 5.iFimn&AtFinhaM IbnMM Ifriom tw 01 wrtact that help in adhesion to Eher GBIIS and augn BKlArai ch remaMma or nuchntri I h*M Mm ti DHA fno

8、ivail*m crnMnwcl inki a pACkrn DNA is ttwa cocte tial dects al gwrtn end8i Penph$m Nudws s!i Cell wall GiycccalyxA Cdolirig m laIF Oi iTiclacdlM ni&DfTuil TO Ibo l wal.H wnm pgcx and f*c*por Knctons BtwownwAni enpori of Ih4 Cfll rne rnfcrane that Mds 9*The cytetplasm dnd mcrMses suftecfl VM.Pfur fto

9、ngiiNk!hoNw 0tiMdi tn t winters M DMA io Etc cits nd in teaacfiQ&lQrk HgkflrQniHlv WE nytnwtai tMisA 1ST.proGpnala w ycoQsn deputed m dense cratals or；paslides that cao be lapped when rmst&M C*4 w*lk-A cemrilfid tHirirvg lhat pnMd&ruetufsajppon iarsl sha(g Cvfl mMmtrArwA th iTMt ol lt|Xd and ixoHn i

10、hol wjnxHMidB*4 cytjpiwmi and cwirois ne low ol m&lBfiais 4nk Mdd col 17 1215 T4DNA6.1.662.HIV1 23 43.17 1315 dilution pour plate method viable cell count 1：10g90ml155ml451001ml9ml10010410510610710821ml3455033728425-2504.Phyolobous fumariiHalobacterium sal in arum HIVHAVSARSSchizosaccharomyces octos

11、porusT4 Deinococcus radiodurans Phyolobous fumariiHalobacterium salinarumDeinococcus radiodurans Schizosaccharomyces octosporus T4HIVHAVSARS512TTTTT6.412，3417 1415 7.t=0N=102/ml8 h N8=109/mlG.1n?t1X1t2nX2=2nX19 2 G=(t2-ti)/3.322(IgN 8-lgN o)=(8-O)/3.322(lg1O-lg10)=0.34h=20.4min 2.303(lgN 8-lgN o)/(t

12、8-to)=2.O15/h 8nn=3.322(lgN 8-lgN o)=23.3 8.16S r DNA1 16S r DNA2 16S rDNA1.5kbPCR3 16S rDNA16S rDNA91)23470S80S5DNA10.123411.17 1515 12.151513.12 100C 114.123151100.2%2%CK pH300mL 250mL100mL25C100/min200.010.050.10.20.30.40.50.751.01.52.0%3y=a+bx+cx217 1615 16.DNA1.1B2 G-3MRVP4050.2-0.5ml212345612pH 34524042 C3.112512317 1715 456-789101112131415164.1215050103250C300 C0-196 C-253 C（324/1h1000100001001010810955.2012345-1059-11SPFIBDV24 h24 h1IBDV2 ELISAPCR17 1815 1 2 36123 45