流式细胞多色分析精选PPT.ppt

《流式细胞多色分析精选PPT.ppt》由会员分享，可在线阅读，更多相关《流式细胞多色分析精选PPT.ppt（10页珍藏版）》请在淘文阁 - 分享文档赚钱的网站上搜索。

1、流式细胞多色分析流式细胞多色分析23-13536-00 Rev.01第1页，此课件共10页哦Multicolor Analysis StrategiesPlot typesHierarchical gatingPopulation hierarchiesBack gating223-13536-00 Rev.01第2页，此课件共10页哦Use Different Plot TypesHistogramsusing histograms can help discriminate between two populations.Dot Plot:hard to discriminate popu

2、lationsDot Plot:displaying fewer events improves discriminationHistogram:population boundary is evident 323-13536-00 Rev.01第3页，此课件共10页哦Use Different Plot TypesContour Plotsusing contour plots can:Help locate population means.Indicate a need for biexponential scaling.Dot Plot:hard to see populations

3、bunched on axisContour Plot:shows that biexponential display might be needed Biexponential Contour Plot:gives best display of populations 423-13536-00 Rev.01第4页，此课件共10页哦Hierarchical Gating523-13536-00 Rev.01第5页，此课件共10页哦Hierarchical GatingWhat went wrong here?Correct Hierarchy623-13536-00 Rev.01第6页，此

4、课件共10页哦Back GatingResearcher wants to discriminate Tregs based on CD25 and CD127 expression.Need to ensure a gate includes the entire FOXP3+population.723-13536-00 Rev.01第7页，此课件共10页哦Additional Analysis StrategiesBiexponential scalingEvents to displayHinged and offset quadrant gatesDerived(boolean)ga

5、tesSnap-to gatesStatisticsAnalysis templates823-13536-00 Rev.01第8页，此课件共10页哦ICS Data Analysis ResultsInformation that can be retrieved:Number of collected events,number of live CD3+cells%of main populations:%CD3+,CD4+,CD8+%of nave(CD45RA+)and memory(CD45RA)CD4+and CD8+cells%CD25+CD4+cells(Tregs)For the cytokines:%of CD4+and CD8+making 1 or 2 cytokinesAnalysis Exercise923-13536-00 Rev.01第9页，此课件共10页哦ICS Panel Analysis1023-13536-00 Rev.01第10页，此课件共10页哦