gen5酶标仪英文说明书.pdf

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1、Microplate SpectrophotometerEonOperators ManualNotification for Kinetic Assays Utilizing the Continuous Shake FeatureA Gen5 experiment that specifies the following parameters may not run successfully on the Eon:Continuous ShakeKinetic Interval greater than 15 minutesWhen the experiment is initiated,

2、plate shaking will begin but shaking may stop prematurely with noerror message.One suggested workaround is to shorten the kinetic interval.For example,if your desired experimentis 25 kinetic reads with 60 minute intervals,use 100 kinetic reads at 15 minute intervals.Another suggestion is to perform

3、multiple Shake steps and then a Read step with the DiscontinuousKinetic Procedure feature enabled.For example,if your desired experiment looks like this:Do this(this example assumes the Read step takes one minute):Please contact BioTeks Technical Assistance Center with any questions,.2012,BioTek Ins

4、truments,Inc.PN 1021000 Rev C1Page 1 of 1EonEonMicroplate SpectrophotometerMicroplate SpectrophotometerOperators ManualOperators ManualOctober 2012 2012Part Number 1021000Revision CBioTek Instruments,Inc.ii|PrefaceNoticesNoticesBioTekBioTek Instruments,Inc.Instruments,Inc.Highland Park,P.O.Box 998Wi

5、nooski,Vermont 05404-0998 USAAll Rights ReservedAll Rights Reserved 2012,BioTek Instruments,Incorporated.No part of this publication may bereproduced,transcribed,or transmitted in any form,or by any means electronic ormechanical,including photocopying and recording,for any purpose other than thepurc

6、hasers use without written permission of BioTek Instruments,Inc.TrademarksTrademarksBioTek is a registered trademark,and Eon,Gen5,and Take3 and Take3 TrioMicro-Volume Plate are trademarks of BioTek Instruments,Inc.BioCell is atrademark of BioTek Instruments and is patented under U.S.patent number 5,

7、963,318.Microsoft,Windows,and Excel are either registered trademarks or trademarks ofMicrosoft Corporation in the United States and/or other countries.All other trademarks are the property of their respective holders.Restrictions and LiabilitiesRestrictions and LiabilitiesInformation in this documen

8、t is subject to change and does not represent acommitment by BioTek Instruments,Inc.Changes made to the information in thisdocument will be incorporated in new editions of the publication.No responsibility isassumed by BioTek for the use or reliability of software or equipment that is notsupplied by

9、 BioTek or its affiliated dealers.BioTek Instruments,Inc.Contents|iiiContentsContentsNotices.iiContact Information.vRevision History.viDocument Conventions.viiIntended Use Statement.viiQuality Control.viiiWarranty and Product Registration.viiiRepackaging and Shipping.viiiWarnings.i xHazards.i xPreca

10、utions.xCE Mark.xiElectromagnetic Interference and Susceptibility.xiiUser Safety.xiiiSafety Symbols.xivChapter 1:Introduction.1Chapter 1:Introduction.1Product Description.2Package Contents.3Optional Accessories.3Product Support and Service.4Chapter 2:Installation.Chapter 2:Installation.5 5Product Re

11、gistration.61:Unpack and Inspect the Instrument.62:Remove the Microplate Carrier Shipping Bracket.73:Select an Appropriate Location.84:Connect the Power Supply.95:Connect the Host Computer.106:Install Gen5 on the Host Computer.107:Turn on the Reader.108:Establish Communication.129:Run a System Test.

12、13Operational/Performance Qualification.14Getting Started with Gen5.14Repackaging and Shipping.15Chapter 3:Instrument QualificationChapter 3:Instrument Qualification.19.19Overview.20IQ/OQ/PQ.20Recommended Qualification Schedule.21System Self-Test.22Absorbance Plate Test.26Absorbance Liquid Tests.31E

13、on Operators Manualiv|PrefaceChapter 4:Preventive MaintenanceChapter 4:Preventive Maintenance.41.41Overview.42Required Materials.42Warnings and Precautions.42Routine Cleaning Procedure.43Decontamination.44Appendix A:Specifications.Appendix A:Specifications.4545General Specifications.46Read Specifica

14、tions.47Appendix B:Error Codes.49Appendix B:Error Codes.49Overview.50General Errors.51Fatal Errors.58A A ppendix C:Instrument Dimensionsppendix C:Instrument Dimensions.59.59Index.63Index.63BioTek Instruments,Inc.Contact Information|vContact InformationContact InformationSee also Product Support&Serv

15、iceProduct Support&Service on page 4.BioTekBioTek Instruments,Inc.Instruments,Inc.Highland Park,P.O.Box 998Winooski,Vermont 05404-0998 USACustomer Service and SalesCustomer Service and SalesInternet:Phone:Fax:E-Mail:888-451-5171(toll free in the U.S.)802-655-4740(outside the U.S.)802-655-Service/TAC

16、Service/TACPhone:800-242-4685(toll free in the U.S.)802-655-4740(outside the U.S.)Fax:802-654-0638E-Mail:European Coordination Center/Authorized EuropeanEuropean Coordination Center/Authorized EuropeanRepresentativeRepresentativeBioTek Instruments GmbHKocherwaldstrasse 34D-74177 Bad FriedrichshallGe

17、rmanyInternet:www.biotek.dePhone:Fax:E-Mail:+49(0)7136 9680+49(0)7136 968 111infobiotek.deEon Operators Manualvi|PrefaceRevision HistoryRevision HistoryRevRevADateDate8/2011ChangesChangesInitial release to ProductionB 6/2012Preface:Preface:Updated the Intended Use Statement.Added Service andAccessor

18、ies hazard warnings.Added Spare Parts precaution.Updatedthe CE Mark information to include EN 61010-2-081 and EN 61010-2-101.Added(if labeled for this use)to Directive 98/79/EC:In Vitro Diagnostics.Ch.3 Instrument Qualification:Ch.3 Instrument Qualification:Simplified the Absorbance Plate Testand li

19、quid tests.C 10/2012Preface:Preface:Updated the CE Mark information to include EN 61010-2-010.Updated the Intended Use Statement to include the“optional cuvetteport”.BioTek Instruments,Inc.Document Conventions|viiDocument ConventionsDocument ConventionsSee alsoSafety SymbolsSafety Symbols on page xi

20、v.This icon calls attention to importantsafetysafety notes.Warning!Warning!AWarningWarning indicates the potential for bodily harm andtells you how to avoid the problem.ACautionCaution indicates potential damage to the instrumentand tells you how to avoid the problem.BoldBold text is primarily used

21、for emphasis.CautionCautionNote:Note:This icon calls attention toimportant informationimportant information.Intended Use StatementIntended Use StatementThe Eon is a single-channel,automated,benchtop,general-purpose microplatespectrophotometer that performs optical density measurements of samples in

22、a microplateformat and optional cuvette port.The performance characteristics of the data reductionsoftware have not been established with any laboratory diagnostic assay.The user mustevaluate this instrument and(if used)PC-based software in conjunction with their specificassay(s).This evaluation mus

23、t include the confirmation that performance characteristicsfor the specific assay(s)are met.If the instrument has an“IVD”label it may be used for clinical and non-clinical purposes,including research&development.If there is no such label the instrument may only beused for research&development or oth

24、er non-clinical purposes.Eon Operators Manualviii|PrefaceQuality ControlQuality ControlIt is considered good laboratory practice to run laboratory samples according toinstructions and specific recommendations included in the package insert for the test to beconducted.Failure to conduct Quality Contr

25、ol checks could result in erroneous test data.Warranty and Product RegistrationWarranty and Product RegistrationTake a moment to review the warranty information that shipped with your product.Please also register your product with BioTek to ensure that you receive importantinformation and updates ab

26、out the product(s)you have purchased.You can register onlinethrough the Customer Resource Center(CRC)at or by calling(888)451-5171 or(802)655-4740.Repackaging and ShippingRepackaging and ShippingIf you need to ship the instrument to BioTek for service or repair,contact BioTek for aReturn Materials A

27、uthorization(RMA)Return Materials Authorization(RMA)number,and be sure to use the original packing materials.Otherforms of commercially available packaging are not recommendedand can void the warranty.If the original packing materials havebeen damaged or lost,contact BioTek for replacement packing.B

28、ioTek Instruments,Inc.Warnings|ixWarningsWarningsOperate the instrument on a level,stable surface away from excessive humidity.Bright sunlight or strong incandescent light can reduce the linear performancerange of the instrument.Measurement values may be affected by extraneous particles(such as dust

29、)in themicroplate wells or cuvettes.A clean work area is necessary to ensure accuratereadings.When operated in a safe environment according to the instructions in thisdocument,there are no known hazards associated with the instrument.However,the operator should be aware of certain situations that co

30、uld result in seriousinjury;these may vary depending on the instrument model.SeeHazardsHazardsandPrecautionsPrecautions.HazardsHazardsThe following hazard warnings are provided to help avoid injury:Warning!Internal Voltage.Warning!Internal Voltage.Always turn off the power switch and unplug thepower

31、 supply before cleaning the outer surface of the instrument.Warning!Power Rating.Warning!Power Rating.The instruments power supply must be connected toa power receptacle that provides voltage and current within the specified ratingfor the system.Use of an incompatible power receptacle may produce el

32、ectricalshock and fire hazards.Warning!Electrical Grounding.Warning!Electrical Grounding.Never use a plug adapter to connect primarypower to the external power supply.Use of an adapter disconnects the utilityground,creating a severe shock hazard.Always connect the power supply directlyto an appropri

33、ate receptacle with a functional ground.Warning!Service.Warning!Service.Only qualified technical personnel should perform serviceprocedures on internal components.Warning!Accessories.Warning!Accessories.Only accessories which meet the manufacturersspecifications shall be used with the instrument.War

34、ning!Liquids.Warning!Liquids.Avoid spilling liquids on the reader;fluid seepage intointernal components creates a potential for shock hazard or instrument damage.If a spill occurs while a program is running,abort the program and turn off theinstrument.Wipe up all spills immediately.Do not operate th

35、e instrument ifinternal components have been exposed to fluid.Eon Operators Manualx|PrefaceWarning!Unspecified Use.Warning!Unspecified Use.Failure to operate this equipment according to theguidelines and safeguards specified in this manual could result in a hazardouscondition.Warning!Software Qualit

36、y Control.Warning!Software Quality Control.The operator must follow themanufacturers assay package insert when modifying software parameters andestablishing reading methods.Failure to conduct quality control checks couldFailure to conduct quality control checks couldresult in erroneous test data.res

37、ult in erroneous test data.Warning!Reader Data Reduction ProtocolWarning!Reader Data Reduction Protocol.No limits are applied to the rawabsorbance data.All information exported via computer control must bethoroughly analyzed by the operator.Warning!Potential Biohazards.Warning!Potential Biohazards.S

38、ome assays or specimens may pose abiohazard.Adequate safety precautions should be taken as outlined in the assayspackage insert.Always wear safety glasses and appropriate protective equipment,such as chemically resistant rubber gloves and apron.PrecautionsPrecautionsThe following precautions are pro

39、vided to help avoid damage to the instrument:Caution:Service.Caution:Service.The instrument should be serviced by BioTek-authorizedservice personnel.Only qualified technical personnel should perform serviceprocedures on internal components.Caution:Spare PartsCaution:Spare Parts.Only approved spare p

40、arts should be used formaintenance.The use of unapproved spare parts and accessories may result in aloss of warranty and potentially impair instrument performance or cause damageto the instrument.Caution:Environmental ConditionsCaution:Environmental Conditions.Do not expose the instrument totemperat

41、ure extremes.For proper operation,ambient temperatures shouldremain with the range listed in theSpecificationsSpecifications section.Performance may beadversely affected if temperatures fluctuate above or below this range.Storagetemperature limits are broader.Caution:Sodium Hypochlorite.Caution:Sodi

42、um Hypochlorite.Do not expose any part of the instrument tothe recommended diluted sodium hypochlorite solution(bleach)for more than 20minutes.Prolonged contact may damage the instrument surfaces.Be certain torinse and thoroughly wipe all surfaces.Caution:Power Supply.Caution:Power Supply.Only use t

43、he power supply shipped with the instru-ment.Operate this power supply within the range of line voltages listed on it.Caution:Disposal.Caution:Disposal.This instrument contains printed circuit boards and wiringwith lead solder.Dispose of the instrument according to Directive 2002/96/EC,“on waste ele

44、ctrical and electronic equipment(WEEE),”or local ordinances.BioTek Instruments,Inc.CE Mark|xiCaution:Warranty.Caution:Warranty.Failure to follow preventive maintenance protocols mayvoid the warrantyvoid the warranty.Caution:Shipping Hardware.Caution:Shipping Hardware.All shipping hardware(e.g.,carri

45、er shippingbracket)must be removed before operating the instrument and reinstalled beforerepackaging the instrument for shipment.Caution:Electromagnetic EnvironmentCaution:Electromagnetic Environment.Per IEC 61326-2-6 it is the usersresponsibility to ensure that a compatible electromagnetic environm

46、ent for thisinstrument is provided and maintained in order that the device will perform asintended.Caution:Electromagnetic CompatibilityCaution:Electromagnetic Compatibility.Do not use this device in closeproximity to sources of strong electromagnetic radiation(e.g.,unshieldedintentional RF sources)

47、,because these may interfere with the proper operation.CE MarkCE MarkBased on the testing described below and information containedBased on the testing described below and information containedherein,this instrument bears the CE mark.herein,this instrument bears the CE mark.Refer to the Declaration

48、of Conformity for more specific information.Directive 2004/108/EC:Electromagnetic CompatibilityDirective 2004/108/EC:Electromagnetic CompatibilityEmissionsClass AEmissionsClass AThe system has been type-tested by an independent,accredited testing laboratoryand found to meet the requirements of EN 61

49、326-1:Class A for Radiated Emissionsand Line Conducted Emissions.Verification of compliance was conducted to thelimits and methods of EN 55011 (CISPR)Class A.In a domestic environment itmay cause radio interference,in which case you may need to mitigate theinterference.ImmunityImmunityThe system has

50、 been type-tested by an independent,accredited testing laboratoryand found to meet the requirements of EN 61326-1 and EN 61326-2-6 forImmunity.Verification of compliance was conducted to the limits and methods ofthe following:EN 61000-4-2,Electrostatic DischargeEN 61000-4-3,Radiated EM FieldsEN 6100