生物化学与分子生物学ppt图片素材scienceslide省公共课一等奖全国赛课获奖课件.pptx

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1、第1页第2页Antibody Toolkit(IgG)第3页 helixVimentinActin and myosinCoiled-coilMicrotubule6 nm10 nm25 nm第4页第5页第6页第7页Nucleosomes 2第8页第9页ACACDDR1DDR1DDR176123454356217CD45PTP第10页Metabolism of 5-fluorouracil(5-FU)5-FUCarac,Efudex5-Fluorouridine5-FUMP5-FdUMPUridine phosphorylase5-FluorodeoxyuridineThymidine pho

2、sphorylaseOrotatephosphoribosyltransferaseThymidine kinase5-FUTP5-FdUTPRNADNA5-nucleotidase5-nucleotidaseUridine kinaseATPADPATPADPPRPP第11页Western Blotting1.Resolve protein samples on SDS-PAGE6.Incubate the blot with chemiluminescent HRP substrate and expose to film 2.Electrophoretically transfer fractionated proteins from gel onto PVDF membrane3.Block the membrane with neutral protein (BSA or milk casein)4.Incubate the membrane with primary antibody specific to target protein5.Incubate the membrane with HRP-labeled secondary antibody specific to primary antibody第12页